Dr. Singh's Publications

 

 

Research Publications in Peer-Reviewed Journals

Books/Editorship

Research Publications in Book/Symposium Volume Chapters

Publications Related to Teaching

Publication in Popular journal/magazines

Patents

Dissertations

Thesis

Presentations/Abstracts















 

Research Publications in Peer-Reviewed Journals

 

  1. Blanchette, B.N. and Singh, B.R. (2002) Isolation and Characterization of the Glutathione S-Transferase Isozyme Q1 from the Northern Quahog Mercinaria mercinaria. J. Protein Chem., in press.
     

  2. Ivanova, D. G., Sarkar, H. K. and Singh, B. R. (2002) Cloning, expression and biological activity of recombinant alpha-cinnamomin: Toxicity to cranberry and other plant species. J. Natural Toxins, in press
     

  3. Cai, S. and Singh, B. R. (2001) Role of disulfide-cleavage induced molten globule state of type A botulinum neurotoxin in its endopeptidase activity. Biochemistry, 40, 15327-15333.
     

  4. Puffer, E. B., Lomneth, R. B., Sarkar, H. K., and Singh, B. R. (2001) Differential roles of developmentally distinct SNAP-25 isoforms in the neurotransmitter release process. Biochemistry 40, 9374-9378.
     

  5. Cai, S. and Singh, B. R. (2001) A Correlation between Differential Structural Features and the Degree of Endopeptidase Activity of Type A Botulinum Neurotoxin in Aqueous Solution, Biochemistry, accepted for publication.
     

  6. Zachariah, V. N., Walsh-Sayles, N. and Singh, B. R. (2001) Isolation, Purification and Characterization of Glutathione-S-Transferase from Oat (Avena sativa) Seedlings. J. Protein Chem., in press.
     

  7. Li, L. and Singh, B. R. (2000) Spectroscopic analysis of pH-induced changes in the molecular features of type A Botulinum neurotoxin light chain. Biochemistry, 39, 6466-6474.
     

  8. Li and Singh, B. R. (2000) Role of zinc binding in type A Botulinum neurotoxin light chain’s toxic structure. Biochemistry 39, 10581-10586.
     

  9. Singh, B. R. (2000) Intimate details of the most poisonous poison. Nature Struct. Biol. 7, 617-619.
     

  10. Li, B., Qian, X., Caruso, F. L., Singh, B. R. and Sarkar, H. K. (2000) Cloning and sequence analysis of a Phytophthora cinnamomi gene which encodes for cinnamomin, a toxin with implications in root rot of cranberry. J. Natural Toxins 9, 113-123.
     

  11. Li, L., Binz, T., Niemann, H. and Singh, B. R. (2000) Probing the mechanistic role of glutamate residue in the HExxH zinc-binding motif of type A Botulinum neurotoxin light chain. Biochemistry 39, 2399-2405.
     

  12. Sharma, S. K. and Singh, B. R. (2000) Immunological Properties of Hn-33 purified from Type A Clostridium Botulinum. J. Nat. Toxin, in press.
     

  13. Li, L. and Singh, B. R. (1999) High level expression, purification and characterization of recombinant type A Botulinum neurotoxin light chain. Protein Expression Purification, 17, 339-344.
     

  14. Fu, F.-N. and Singh, B. R. (1999) Calcein permeability of liposome's mediated by type A Botulinum neurotoxin and its light and heavy chains. J. Protein Chem. 18, 701-707.
     

  15. Cai, S. and Singh, B. R. (1999) Identification of b-turn and random coil amide III infrared bands for secondary structure estimation of proteins. Biophysical Chem. 80, 7-20.
     

  16. Hadfield, A., Petsko, G., Lindo, P. and Singh, B. R. (1999) Preliminary crystallographic studies of a protease resistant Botulinum neurotoxin associated protein Hn-33. Acta Crystallographic D: Biological Crystallography 55, 1237-1239.
     

  17. Shukla, H. D. and Singh, B. R. (1999) Identification of DnaJ-like chaperone in Clostridium Botulinum type A. J. Protein Chem. 18, 695-700.
     

  18. Cai, S., Sarkar, H. K. and Singh, B. R. (1999) Enhancement of the endopeptidase activity of Botulinum neurotoxin by its associated proteins and dithiothreitol. Biochemistry, 38, 6903-6910.
     

  19. Li, L. and Singh, B. R. (1999) Structure-function relationship of clostridial neurotoxins. Toxin Reviews, 18, 95-112.
     

  20. Lebeda, F. J. and Singh, B. R. (1999) Membrane channel activity and translocation of tetanus and botulinum neurotoxins. Toxin Reviews, 18,
    45-76.
     

  21. Sharma, S. K., Fu, F.-N. and Singh, B. R. (1999) Molecular properties of a hemagglutinin purified from type A botulinum neurotoxin complex. J. Protein Chem. 18, 29-38.
     

  22. Blanchette, B. N. and Singh, B. R. (1999) Purification and characterization of the glutathione-S-transferases from the northern quahog, Mercenaria mercinaria. J. Marine Biotechnol., 1, 74-80.
     

  23. Li, L. and Singh, B. R. (1999) In vitro translation of type A Clostridium botulinum neurotoxin heavy chain and analysis of its binding to rat synaptosomes. J. Protein Chem., 18, 89-95.
     

  24. Li, L. and Singh, B. R. (1998) Isolation of synaptotagmin as a receptor for types A and E botulinum neurotoxin and analysis of their comparative binding using a new microtiter plate assay. J. Natural Toxins 7, 215-226.
     

  25. Sharma, S. K. and Singh, B. R. (1998) Hemagglutinin binding protection of botulinum neurotoxin from proteolysis. J. Natural Toxins 7, 239-253.
     

  26. Fu, F. -N., Lomneth, R. B., Cai, S. and Singh, B. R. (1998) Role of zinc in the toxic structure and activity of botulinum neurotoxin. Biochemistry 37, 5267-5278.
     

  27. Li, B., Qian, X. , Sarkar, H. K. and Singh, B. R. (1998) Molecular characterization of type E C. botulinum and comparison with other types of Clostridium botulinum. Biochim. Biophys. Acta. 1395, 21-27.
     

  28. Fu, F. -N., Sharma, S. K. and Singh, B. R. (199871 30ino acid side chains and) A protease-resistant novel hemagglutinin purified from type A Clostridium botulinum. J. Protein Chem. 17, 53-60.
     

  29. Ge, J. and Singh, B. R. (1996) Physico-chemical characterization of glutathione-s-transferase purified from oyster, Crassostrea virginica. J. Marine Biotech. 4, 150-154.
     

  30. Singh, B. R. (1996) Current frontiers of natural toxins. J. Natural Toxins 5, 127-13.
     

  31. Singh, B. R., Li, B. and Read, D. (1995) Botulinum versus tetanus neurotoxins: Why is botulinum neurotoxin a food poison but not tetanus? Toxicon 33, 1541-1547.
     

  32. Singh, B. R., Barcomb-Caddle, L. A., Fu, F.-N. and Li, B. (1996) Gene probe-based detection of type E botulinum neurotoxin binding protein using polymerase chain reaction. Toxicon, 34, 737-742.
     

  33. Singh, B. R., Lopes, T. and Silvia, M. A. (1996) Immunochemical characterization of type A botulinum neurotoxin in its purified and complexed forms. Toxicon 34, 267-275.
     

  34. Hu, Y. and Singh, B. R. (1995) Comparative surface adsorption behavior of high and low density lipoproteins as analyzed by FT-IR/ATR spectroscopy. Applied Spectroscopy 49, 384-397.
     

  35. Singh, B. R., Foley, J. and Catherine Lafontaine (1995) Physico-chemical characterization of the botulinum neurotoxin binding protein from type E botulinum producing Clostridium botulinum. J. Protein Chem, 14, 7-18.
     

  36. Singh, B. R., Fu, F. -N. and Ledoux, D. N. (1994) Crystal versus solution structure of superantigenic staphylococcal enterotoxins. Nature Structural Biology 1, 358-360.
     

  37. Poirier, M. P., Lopes, T. and Singh, B. R. (1994) Use of an optical fiber-based biosensor to study the interaction of blood proteins with solid surfaces. Applied Spectroscopy 48, 867-870.
     

  38. Fu, F.-N., DeOliveira, D. B., William R. Trumble, Hemanta K. Sarkar and Singh, B. R. (1994) Secondary structure estimation of proteins using the amide III region of Fourier transform infrared spectroscopy: Application to analyze calcium-binding-induced structural changes in calsequestrin. Applied Spectroscopy 48, 1432-1441
     

  39. Be, X., Fu, F. -N. and Singh, B. R. (1994) Hydrophobic moment analysis of amino acid sequences of botulinum and tetanus neurotoxins to identify functional domains. J. Natural Toxins. 3, 49-68.
     

  40. Ledoux, D. N., Be, X. and Singh, B. R. (1994) Quaternary structure of botulinum and tetanus neurotoxins as probed by chemical cross-linking and native gel electrophoresis. Toxicon, 32, 1095-1104.
     

  41. Fu, F.-N., Fuller, M. P. and Singh, B. R. (1993) Use of FT-IR/attanuated total reflectance spectroscopy for the study of surface adsorption of proteins. Applied Spectrosc. 47, 98-102.
     

  42. Ogert, R. A., Brown, J. E., Singh, B. R., Shriver-Lake, L. and Ligler, F. S. (1992) Detection of Clostridium botulinum toxin A using a fiber optic-based biosensor. Anal. Biochem. 205, 306-312.
     

  43. Singh, B. R., Fuller, M. P. and DasGupta, B. R. (1991) Botulinum neurotoxin type A: Structure and interaction with micellar concentration of SDS determined by FT-IR spectroscopy. J. Protein Chem. 10, 637-649.
     

  44. Singh, B. R. and Fuller, M. P. (1991) FT-IR in combination with the attenuated total reflectance technique: A very sensitive method for the structural analysis of polypeptides. Appl. Spectrosc. 45, 1017-1021.
     

  45. Singh, B. R., Gimenez, J. A. and DasGupta, B. R. (1991) Comparative molecular topography of botulinum neurotoxins from Clostridium botulinum and C. butyricum. Biochim. Biophys. Acta 1077, 119-126.
     

  46. Singh, B. R., Sarvari, E., Agnihotri, A. and Singhal, G. S. (1991) Lincomycin induced alteration in the contents of chlorophyll-protein complexes of maize chloroplasts and its effect on the temperature induced spectral changes. Physiol. Plant 181, 393-398.
     

  47. Singh, B. R., Wasacz, F. M., Strand, S., Jakobsen, R. J. and DasGupta, B. R. (1990) Comparative structural analysis of botulinum neurotoxins types A and E using Fourier transform infrared and circular dichroic spectroscopies. J. Protein Chem.7, 705-713.
     

  48. Singh, B. R. and DasGupta, B. R. (1990) Conformational changes associated with nicking and activation of the type E botulinum neurotoxin. Biophysical chemistry 38, 123-130.
     

  49. Singh, B. R., Fuller, M. P. and Schiavo, G. (1990) Molecular structure of tetanus neurotoxin as revealed by circular dichroism and Fourier transform infrared spectroscopy. Biophysical Chem. 36, 155-166.
     

  50. Singh, B. R. (1990) Identification of specific domains of botulinum and tetanus neurotoxins. Toxicon 28, 992-996.
     

  51. Singh, B. R. and Song, P. S. (1990). Phytochrome and protein phosphorylation. Photochem. Photobiol. 52, 249-254.
     

  52. Singh, B. R. and Song, P. S. (1990) A differential molecular topography of the Pr and Pfr forms of native phytochrome probed by dynamic fluorescence quenching. Planta 181, 263-267.
     

  53. Singh, B. R. and Singhal, G. S. (1990) Spectral changes in barley chloroplast membranes induced by a combination of a membrane perturbant and temperature. Biochem. Physiol. Pflanzen 186, 19-29.
     

  54. Singh, B. R. and DasGupta, B. R. (1989) Changes in the molecular environments of Trp and Tyr residues of the light and heavy chains of type A botulinum neurotoxin following their separation. Biophysical Chem. 34, 259-267.
     

  55. Singh, B. R. and Betley, M. J. (1989) Comparative structural analysis of staphylococcal enterotoxins A and E. J. Biol. Chem. 264, 4404-4411.
     

  56. Singh, B. R. DasGupta, B. R. (1989) Structure of light and heavy chain subunits of type A botulinum neurotoxin analyzed by circular dichroism and fluorescence measurements Mol. Cell. Biochem. 85, 67-73.
     

  57. Singh, B. R. and DasGupta, B. R. (1989) Molecular topography and secondary structure comparisons of botulinum neurotoxin types A, B and E. Mol. Cell. Biochem. 86, 87-95.
     

  58. Singh, B. R. and DasGupta, B. R. (1989) Molecular differences between type A botulinum neurotoxin and its toxoid. Toxicon 27, 403-410.
     

  59. Singh, B. R., Song, P. S., Eilfeld, P. and Rudiger, W. (1989) Differential exposure of aromatic amino acids in the red-light-absorbing and far-red-light-absorbing forms of 124 kDa oat phytochrome. Eur. J. Biochem. 184, 715-721.
     

  60. Song, P. S., Singh, B. R., Tamai, N., Yamazaki, T., Yamazaki, I., Tokutami, S. and Furuya, M. (1989) Primary photoprocesses of phytochrome: Picosecond fluorescence kinetics of oat and pea phytochrome. Biochemistry 28, 3265-3271.
     

  61. Singh, B. R. (1989) Energy transfer between tryptophan and chlorophyll a of peridinin-chlorophyll a-protein. Biochem. Physiol. Pflanzen 184, 205-211.
     

  62. Singh, B. R., Choi, J. K., Kwon, T. I. and Song, P. S. (1989) Use of bilirubin oxidase for probing chromophore topography in tetrapyrrolic proteins. J. Biochem. Biophys. Meth. 18, 135-148.
     

  63. Singh, B. R. and Song, P. S. (1989) Interaction between native oat phytochrome and tetrapyrroles. Biochim. Biophys. Acta 996, 62-69.
     

  64. Singh, B. R., Chai, Y. G., Robertson, D. T. and Song, P. S. (1989) A photoreversible phytochrome affinity column chromatography for putative phytochrome receptor studies. J. Biochem. Biophys. Meth. 18,
    105-112.
     

  65. Singh, B. R., Choi, J. K., Kim, I. S. and Song, P. S. (1989) Binding of 124 kDa phytochrome to liposomes and chloroplasts. Physiol. Plant. 76, 319-325.
     

  66. Singh, B. R. and Singhal, G. S. (1989) Temperature-induced spectral changes of chloroplasts. Biochem. Physiol. Pflanzen 184, 193-203.
     

  67. Singh, B. R. and Singhal, G. S. (1989) Role of surface proteins in temperature-induced spectral changes in barley chloroplast membranes. Biochem. Physiol. Pflanzen 185, 1-10.
     

  68. Singh, B. R. and Shaw, R. W. (1988) Selective inhibition of oat glutathione-S-transferase by tetrapyrroles. FEBS Lett. 234, 379-382.
     

  69. Singh, B. R., Evenson, M. L. and Bergdoll, M. S. (1988) Structural analysis of staphylococcal enterotoxins B and C1 using circular dichroism and fluorescence spectroscopy. Biochemistry 27, 8735-8741.
     

  70. Singh, B. R., Kokan-Moore, N. P. and Bergdoll, M. S. (1988) Molecular topography of toxic shock syndrome toxin-1 as revealed by spectroscopic measurements. Biochemistry 27, 8730-8735.
     

  71. Singh, B. R., Chai, Y. G., Song, P. S., Lee, J. and Robinson, G. W. (1988) A photoreversible conformational change in 124 kDa phytochrome. Biochim. Biophys. Acta 936, 395-405.
     

  72. Chai, Y. G., Singh, B. R., Song, P. S., Lee, J. and Robinson, G. W. (1987) Purification and spectroscopic properties of 124-kDa oat phytochrome. Anal. Biochem. 163, 322-330.
     

  73. Singh, B. R. (1988) Role of membrane organization in the Mg2+-mediated regulation of excitation energy transfer in barley chloroplast membranes. J. Biol. Phys. 16, 33-36.
     

  74. Singh, B. R. and Singhal, G. S. (1985) Temperature-induced absorbance changes in developing barley chloroplasts. Physiol. Plant. 65, 294-298.
     

  75. Singh, B. R. and Singhal, G. S. (1984) Effect of aliphatic alcohols on the temperature-induced absorption changes in barley chloroplasts. Photobiochem. Photobiophys. 8, 73-84.

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Books/Editorship

 

  1. Singh, B. R. and Tu, A. (1996) Natural Toxins II: Structure, Mechanism of Action and Detection, Plenum Press, New York.
     
  2. Singh, B. R. (2000) Infrared Analysis of Peptides and Proteins. ACS Books, Washington, D. C.


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Research Publications in Book/Symposium Volume Chapters :

  1. Singhal, P., Zhang, J., Michel, H. E. and Singh, B. R. (2002) Fish-Quality Analysis Using Artificial Neural Networks and Spectroscopic Data. IASTED International Conference on Artificial Intelligence and Soft Computing (ASC 2002), which will be held July 17 to July 19, 2002, in Banff, Canada.
     

  2. Singh, B. R. (2002) Molecular basis of the unique endopeptidase activity of botulinum neurotoxin. In: Scientific and Therapeutic Aspects of Botulinum Toxin (M. F. Brin, J. Jankovic, and M. Hallet, eds.), Lippincott Williams and Wilkins, Philadelphia. Chapter 7, in press.
     

  3. Cai, S. and Singh, B. R. (1999) Determination of the secondary structure of proteins from Amide I and Amide III infrared bands using partial least square method. In: Infrared Analysis of Peptides and Proteins (B. R. Singh, Ed.), ACS Books, Washington, D. C. pp. 117-129.
     

  4. Singh, B. R. (1999) Basic aspects of the technique and applications of infrared spectroscopy of peptides and proteins. In: Infrared Analysis of Peptides and Proteins (B. R. Singh, Ed.), ACS Books, Washington,
    D. C. pp. 2-53.
     

  5. Singh, B. R. (1999) Biomedical and toxico-chemical aspects of botulinum neurotoxins (Editorial). Toxin Reviews, 18, vii-x.
     

  6. Boulanger, Jr., R. B. and Singh, B. R. (1997) Phytochrome-mediated regulation of anthocyanin biosynthesis in cranberry plants. The Commonwealth Undergraduate Review 2, 19-21.
     

  7. Zachariah, V. T. and Singh, B. R. (1997) Effect of plant greening and senescence on the enzymatic activity of glutathione-S-transferase, a detoxifying enzyme. The Commonwealth Undergraduate Review 2, 31-35.
     

  8. Singh, B. R. and Silvia, M. A. (1996) Detection of botulinum neurotoxins using optical fiber-based biosensor. In: Natural Toxins II: Structure, Mechanism of Action and Detection (Singh, B. R. and Tu, A., eds.), Plenum Press, New York. pp. 499-508.
     

  9. Singh, B. R. (1996) Critical aspects of bacterial protein toxins. In: Natural Toxins II: Structure, Mechanism of Action and Detection (Singh, B. R. and Tu, A., eds.), Plenum Press, New York. pp.63-84.
     

  10. Singh, B. R. (1996) Role of Botulinum Complexing Proteins in the Detection of Botulinum Neurotoxins. Proceedings of the Scientific Conference on Chemical and Biological Defense, Aberdeen Proving Ground, Edgewood, MD. pp. 87-94.
     

  11. Singh, B. R., Ledoux, D. N. and Fu, F. -N. (1994) An analysis of the protein structure of botulinum and tetanus neurotoxins to understand the molecular basis of membrane channel formation. In : Advances in Venom and Toxin Research (N. H. Tan, S.L. Go, V. Thambyrajah and N. Azila, eds.), Malaysisan Society on Toxinology, Kuala Lupur, Malaysia,
    pp. 103-108.
     

  12. Singh, B. R. DeOliveira, D. B. Fu, F.-N. and Fuller, M. P. (1993) Fourier transform infrared analysis of amide III bands of proteins for the secondary structure estimation. Proceedings of Biomolecular Spectroscopy III, SPIE Volume 1890, 47-55.
     

  13. Singh, B. R. and Poirier, M. P. (1993) Interaction of high density and low density lipoproteins to solid surfaces coated with cholesterol as determined by an optical fiber-based biosensor.Proceedings of Fiber Optic Sensors in Medical Diagnostics, SPIE Volume 1886, 27-34.
     

  14. Singh, B. R. (1993) Structure-function relationship of botulinum and tetanus neurotoxins. In: Procedings of the International Conference on Botulinum and Tetanus Neurotoxins: Neurotransmission and Biomedical Aspects (B. R. DasGupta, ed.), Plenum Press. pp. 377-392.
     

  15. Doyle, J. and Singh, B. R. (1992) Molecular basis of of low pH-dependent membrane translocation of botulinum and tetanus neurotoxins. In: Procedings of the International Conference on Botulinum and Tetanus Neurotoxins: Neurotransmission and Biomedical Aspects (B. R. DasGupta, ed.), Plenum Press. 231-235.
     

  16. Singh, B. R. and Be, X.-H. (1992) Use of sequence hydrophobic moment to analyze the membrane interacting domains of botulinum, tetanus and other toxins. In: Techniques in Protein Chemistry III (R. H. Angeletti, ed.), Academic Press, New York. pp. 373-383.
     

  17. Singh, B. R., Fu, F.-N. and Fuller, M. P. (1992) Fourier transform infrared analysis of proteins in terms of detectability, conformation and adsorption density. In: Techniques in Protein Chemistry III (R. H. Angeletti, ed.), Academic Press, New York, pp. 385-398.
     

  18. Singh, B. R. (1991) Plant photomorphogenic photoreceptor phytochrome. Molecular topography and primary events. In: Trends in Bioenergetics and Biotechnological Processes (G. S. Singhal and T. Ramasarma, eds.), Vedams Books International, New Delhi. pp. 159-173.
     

  19. Singh, B. R. and DasGupta, B. R. (1990) Molecular conformations of botulinum neurotoxins. In: Bacterial Protein Toxins (R. Rappuoli et al., Eds.), Gustav Fisher Verlag, Stuttgart-New York, 109-110.
     

  20. Fuller, M. P. and Singh, B. R. (1989) Sampling and resolution enhancement techniques for the infrared analysis of the adsorbed proteins. In: Proceedings of Seventh International Conference on Fourier Transform Spectroscopy, SPIE Volume 1145, 420-422.


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Publications Related to Teaching

  1. Blanchette, B. N. and Singh, B. R. (2000) A Single Protein Research Integrated Advanced Biochemistry Laboratory Course; Spectroscopic Determination of Tyr Exposure and pKa in a Protein. Submitted to Biochemistry and Molecular Biol. Education.
     

  2. Singh, B. R. (2000) The perplexity of teaching a chemistry course to nursing students: possible solutions. Submitted to J. Chem Education.
     

  3. Blanchette, B. N. and Singh, B. R. (2000) A single protein research integrated advanced biochemistry laboratory course; Spectroscopic determination of Tyr side chain pKa. Bochemical Education, 28, 107-109.
     

  4. Singh, B. R. (1999) A single protein research integrated advanced biochemistry laboratory course. I. Course design and general outline. Biochemical Education, 27, 41-44.
     

  5. Singh, B. R. (1999) An effective first day exercise on relevance of chemistry to non-science majors kindles sustained positive student response J. Chem. Education, 76, 1299-1220.
     

  6. Singh, B. R., Wechter, M. A., Y. Hu and C. L. Lafontaine (1998) Determination of caffeine content in coffee using Fourier transform infra-red spectroscopy in combination with attenuated total reflectance technique: A bioanalytical chemistry experiment for biochemists. Biochemical Education, 26, 243-247.
     

  7. Singh, B. R. and Deck, J. C. (1998) Etiquette in departmental seminars. J. Chem. Education 75, 846-848.
     

  8. Singh, B. R. (1995) Relevance of chemistry to non-science majors: Students viewpoints J. Chem. Education. 72, 432-434.

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Publication in Popular journal/magazines

 

  1. Zhou, Yu and Singh, B.R. (2004) Effect of Light on Anthocyanin Levels in Submerged, Harvested Cranberry Fruit/ J. Biomedicine & Biotechnology, June 2004, pp 259-263
     

  2. Sharma, Shashi K. and Singh, B.R.(2004) Enhancement of the Endopeptidase Activity of Purified Botulinum Neurotoxins A and E by an Isolated Component of the Native Neurotoxin Associated Proteins. Biochemistry, Vol. 43, # 16 4791-4798
     

  3. Zhou, Yu and Singh, B.R. (2004) Cloning, high-level expression, single-step purification, and binding activity of His6-tagged recombinant type B botulinum neurotoxin heavy chain transmembrane and binding domain. Revised Oct. 2003, Protein Expression & Purification, 34, 2004, pp 8-16
     

  4. Singh, B.R. and Cai, S. (2004) A Distinct Utility of the Amide III Infrared Band for Secondary Structure Estimation of Aqueous Protein Solutions Using Partial Least Squares Methods. Biochemistry, Vol. 43, #9, 2541-2549
     

  5. Singh, B.R. and Blanchette, B. (2003) An Enzyme Based Dechlorination of a Polychlorinated Biphenyl (PCB) Mixture, Aroclor 1248, Using Clutathione S-Transferases from The Northern Quahog Mercenaria J-Protein Chem. Vol. 22, #4, 377-386
     

  6. Singh, B. R. (2000) Light regulation of anthocyanin (red color pigment) biosynthesis in cranberry plants. 1999 Cranberry Research Compilation: Progress and Final Reports on Cranberry Research (Deziel, G. and Silvia, A.), Cranberry Institute, Wareham, MA. Pp. 506-516.
     

  7. Singh, B. R. (2000) Design of biosensor-based detection of Phythophthora root rot agents. 1999 Cranberry Research Compilation: Progress and Final Reports on Cranberry Research (Deziel, G. and Silvia, A.), Cranberry Institute, Wareham, MA. Pp. 446-451.
     

  8. Boulanger, Jr., R. B. and Singh, B. R. (1999) Anthocyanin and flavonol contents of cranberry fruits as a function of seasonal development and light intensity. 1998 Cranberry Research Compilation: Progress and Final Reports on Cranberry Research (Deziel, G. and Silvia, A.), Cranberry Institute, Wareham, MA. Pp. 313-317.
     

  9. Boulanger, Jr., R. R. and Singh, B. R. (1998) Light regulation of anthocyanin and flavonol biosyntheis in cranberry plants. The Nucleus (Northeastern Section American Chemical Society), 76, 14-18.
     

  10. Li, B., Qian, X., Caruso, F. L., Singh, B. R. and Sarkar, H. K. (1999) Analysis of cinnamomin gene from Phytophthora cinnamomi isolates from cranberry bogs. 1998 Cranberry Research Compilation: Progress and Final Reports on Cranberry Research (Deziel, G. and Silvia, A.), Cranberry Institute, Wareham, MA. Pp. 305-312.
     

  11. Ge, J. and Singh, B. R. (1995) Purification and characterization of glutathione-S-transferase from Oyster. The Nucleus (Northeastern Section American Chemical Society), 73, 10-12.
     

  12. Poirier, M. A. and Singh, B. R. (1994) Enzymatic activity of glutathione-S-transferase in non-aqueous solvents. The Nucleus (Northeastern Section ACS) 72, 14-17.

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Patents

 

  1. Singh, B. R. and Zhang, Z. (1996) Novel proteins within the type E botulinum neurotoxin complex. U.S. Patent Serial No. 60/021,348.
     

  2. Singh, B. R. and Sharma, S. K. (1998) Biologically active hemagglutinin from type A Clostridium botulinum and methods of use. U. S. Patent Serial No. 09/013,411


 

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Dissertations

 

  1. Blanchette, B. N. (2002) An enzyme-based dechlorination of PCBs: Glutathione-S-transferase from the northern quahog, Mercinaria mercinaria, as a promising candidate. Ph.D. Dissertation, UMass Dartmouth-UMass Lowell Joint Ph.D. Program.
     

  2. Cai, S. (2001) Molecular mechanisms of the activation of Type A botulinum neurotoxin endopeptidase. Ph.D. Dissertation, UMass Amherst-UMass Dartmouth Co-Op Ph.D. Program
     

  3. Li, L. (2000) Molecular Basis of the Endopeptidase Activity of Type A Botulinum Neurotoxin. Ph.D. Dissertation, UMass Amherst-UMass Dartmouth Co-Op Ph.D. Program.
     

  4. Fu, F. –N. (1997) Structure and function relationship of Botulinum neurotoxin and a neurotoxin associated protein, Ph.D. Dissertation, UMass Amherst-UMass Dartmouth Co-Op Ph.D. Program.


 

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Thesis

 

  1. Sun, Y. (2000) Determination of protein structure using amide III FT-IR spectroscopy. M. S. Thesis, University of Massachusetts Dartmouth.
     

  2. Lee, D. (1999) Inhibition of the endopeptidase activity of LC of type A botulinum neurotoxin by sodium perborate. M. S. Thesis, University of Massachusetts Dartmouth.
     

  3. Cai, S. (1998) Determination of protein secondary structure using FT-IR spectroscopy. M. S. Thesis, University of Massachusetts Dartmouth.
     

  4. Parikh, S. (1998) Mechanism of botulinum neurotoxin translocation and blockage of neurotransmitter release. M. S. Thesis, University of Massachusetts Dartmouth.
     

  5. Li, B. (1997) A new genomic organization for Clostridium botulinum type E: Does type E progenitor neurotoxin contain only neurotoxin and neurotoxin binding protein? M. S. Thesis, University of Massachusetts Dartmouth.
     

  6. Li, L. (1997) Receptor and mechanism of endocytotic translocation of botulinum neurotoxin. M. S. Thesis, University of Massachusetts Dartmouth.
     

  7. Zhang, Z. (1996) Molecular basis of enzyme thermonstability in non-aqueous solvents. M. S. Thesis, University of Massachusetts Dartmouth.
     

  8. Hu, Y. (1995) Surface Adsorption and structural analysis of high and low density lipoproteins using infrared spectroscopy. M. S. Thesis, University of Massachusetts Dartmouth.
     

  9. Silvia, M. A. (1994) Detection of type E Clostridium botulinum neurotoxin using fiberoptic biosensor. M. S. Thesis, University of Massachusetts Dartmouth.
     

  10. Ledoux, D. N. (1994) Structure-function relationship of clostridial neurotoxins. M. S. Thesis, University of Massachusetts Dartmouth.
     

  11. Be, X. H. (1993) Structure-function relationship of botulinum and tetanus neurotoxins. M. S. Thesis, University of Massachusetts Dartmouth.
     

  12. Fu, F.-N. (1993) Fourier transform infrared spectroscopic analysis of proteins adsorbed on a solid surface. M. S. Thesis, University of Massachusetts Dartmouth.


 

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Presentation Abstracts

 

  1. Cai, S., Sharma, S. K. and Singh, B. R. (2001) Unique Structural Features of Botulinum Neurotoxin Required for its Endopeptidase Activity. Thirty-eighth Annual Meeting of Interagency Botulism Research Coordinating Committee. October 17-19, Easton, MD.
     

  2. Sharma, S. K. and Singh, B. R. (2001) Hn-33 enhances the endopeptidase activity of botulinum neurotoxin A and E against brain synaptosomal SNAP-25. University of Massachusetts Neuroscience Symposium, June 4, Worcester, MA.
     

  3. Nunes, R. B. and Singh, B. R. (2001) Expression, Purification, And Role Of Zinc Binding In The Structure And Function Of A Mutant Recombinant Type A Botulinum Neurotoxin Light Chain. University of Massachusetts Neuroscience Symposium, June 4, Worcester, MA.
     

  4. Rogers, S. A. and Singh, B. R. (2001) Interaction of the Most Poisonous Poison with Nerve Terminals as Examined by Isothermal Titration Calorimetry. University of Massachusetts Neuroscience Symposium, June 4, Worcester, MA.

     

  5. Kang, J. and Singh, B. R. (2001) Expression, Purification, and Structural Analysis of Recombinant Botulinum Neurotoxin Light Chain Mutants to Understand Mechanistics of its Endopeptidase Activity Against SNAP-25. University of Massachusetts Neuroscience Symposium, June 4, Worcester, MA.
     

  6. Puffer, E. B., Lomneth, R. B., Sarkar, Hemanta K. and Singh, B. R. (2001) Differential Roles of Developmentally Distinct SNAP-25 Isoforms in Neurotransmitter Release Process. University of Massachusetts Neuroscience Symposium, June 4, Worcester, MA.
     

  7. Zhou, Y. and Singh, B. R. (2001) Interaction of Botulinum Neurotoxin with Plant Cell System Reveals Neuronal Type of Processes in Plant Tissues. University of Massachusetts Neuroscience Symposium, June 4, Worcester, MA.
     

  8. LaPerriere, E. and Singh, B. R. (2001) Tagging One Of The Most Poisonous Poisons With Synaptotagmin Manufactrured In E. coli. University of Massachusetts Neuroscience Symposium, June 4, Worcester, MA.
     

  9. Kang, J. and Singh, B. R. (2001) Expression, purification, and structural analysis of recombinant Clostridium botulinum light chain mutants. Seventh Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 1-2, 2001.
     

  10. Ivanova, D. G. and Singh, B. R. (2001) Use of FT-IR spectroscopy for the analysis of Phytophthora elicitin-induced changes in plant leaves. Seventh Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 1-2, 2001.
     

  11. LaPerriere, E. L. and Singh, B. R. (2001) Tagging of the most poisonous poison with synaptotagmin manufactured in E. coli. Seventh Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 1-2, 2001.
     

  12. Nunes, R. and Singh, B. R. (2001) Expression, purification, and role of zinc in the structure and function of a mutant recombinant type A botulinum neurotoxin light chain. Seventh Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 1-2, 2001.
     

  13. Rogers, S. and Singh, B. R. (2001) Interaction of the most poisonous poison with nerve terminals as examined by isothermal titration calorimetry. Seventh Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 1-2, 2001.
     

  14. Roy, J. and Puffer, E. B., Sarkar, H. K. and Singh, B. R. (2001) Molecular basis of differential neuronal distribution and function of SNAP-25, a protein with potential use in neuro-regeneration. Seventh Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 1-2, 2001.
     

  15. Sharma, S. K. and Singh, B. R. (2001) Hemagglutinin-33 of type A Clostridium botulinum is stubbornly resistance to denaturation. Seventh Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 1-2, 2001.
     

  16. Singh, B. R., Fan, Q. and Bhalerao, S. (2001) Extraction, preparation and formulation of eco-friendly natural dyes from cranberry fruits. Seventh Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 1-2, 2001.
     

  17. Zhang, J., Ivanova, D. G. and Singh, B. R. (2001) Design of gene probe sensor for Phytophthora cinnamomi. Seventh Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 1-2, 2001.

  18. Zhou, Y. and Singh, B. R. (2001) Characterization of responses of American cranberry plant to light. Seventh Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 1-2, 2001.

  19. Sharma, S. K. and Singh, B. R. (2000) Understanding the molecular mechanism of botulinum endopeptidase activity by examining the process of its activation. Thirty Seventh Annual Meeting of Interagency Botulism Research Coordinating Committee. October 17-20, Asilomar, CA

  20. Cai, S. and Singh, B. R. (2000) Mechanism of botulinum neurotoxin action: partially unfolded state is essential for the endopeptidase activity of botulinum neurotoxin type A. . FASEB J., A1415 # 1538.

  21. Kang, J., Li, L., Li, B. and Singh, B. R. (2000) Genetic mapping of neurotoxin associated proteins of Clostridium botulinum type E. . FASEB J., A1415 # 1537.

     

  22. Li, L. and Singh, B. R. (2000) Role of zinc binding in type A botulinum neurotoxin light chain’s toxic structure. . FASEB J., A1415 # 943.

     

  23. Puffer, E. B. and Singh, B. R. (2000) Cleavage resistant SNAP-25 mutant investigates recognition and binding of botulinum neurotoxins. . FASEB J., A1415 # 942.

     

  24. Sharma, S. K. and Singh, B. R. (2000) Proteolytic stability and endopeptidase activity of botulinum neurotoxin A and E. FASEB J., A1415 # 941.

     

  25. Sharma, S. K. and Singh, B. R. (2000) Hn-33 enhances the endopeptidase activity of botulinum neurotoxin A and E against brain synaptosomal SNAP-25. FASEB J., A1415 # 940.
     

  26. Zhou, Y. and Singh, B. R. (2000) Effect of light on the anthocyanin synthesis of cranberry fruits. FASEB J., A1415 # 597.
     

  27. Yin, H., Boulanger, R. R. and Singh, B. R. (2000) Variation in the anthocyanin and flavonol contents of cranberry fruits as a function of seasonal development and temperature change. FASEB J., A1415 # 596.
     

  28. Zhou, Y. and Singh, B. R. (2000) Induction of cranberry callus for the study of cranberry plant physiology. Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 9-10, 2000.
     

  29. Yin, H. and Singh, B. R. (2000) Seasonal development pattern of individual anthocyanins and flavonols of cranberry fruits as a function of environmental factors. Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 9-10, 2000.
     

  30. Singh, B. R. and Boswell, K. (2000) A tissue specific characterization of the glutathione-S-transferase isozymes from the northern quahog Mercinaria mercinaria. Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 9-10, 2000.

  31. Sharma, S. K. and Singh, B. R. (2000) Proteolytic stability and endopeptidase activity of botulinum neurotoxin complex A and E. Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 9-10, 2000.

  32. Puffer, E., Li., L. and Singh, B. R. (2000) Cleavage resistant SNAP-25 mutant investigates recognition and binding of botulinum neurotoxins. Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 9-10, 2000.

  33. Sharma, S. K. and Singh, B. R. (2000) HN-33 enhances the endopeptidase activity of botulinum neurotoxin A and E against brain synaptosomal SNAP-25. Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 9-10, 2000.

  34. Li, L. and Singh, B. R. (2000) Role of zinc binding in type A botulinum neurotoxin light chain’s toxic structure. Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 9-10, 2000.

  35. Kang, J., Li, B. and Singh, B. R. (2000) Analysis of the P48 gene in Clostridium botulinum type E. Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 9-10, 2000.

  36. Cai, S. and Singh, B. R. (2000) Mechanism of botulinum neurotoxin: molten globule state is essential for the endopeptidase activity of botulinum neurotoxin type A. Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 9-10, 2000.

  37. Blanchette, B. N. and Singh, B. R. (2000) The isolation and characterization of isozyme Q1 from the northern quahog, Mercinaria mercinaria. Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 9-10, 2000.

  38. Ivanova, D. G., Agrawal, T. and Singh, B. R. (2000) Genetic basis and biochemical mechanism of cranberry root rot caused by Phytophthora cinnamomi. Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 9-10, 2000.

  39. Blanchette, B. N., DeSouza, R. and Singh, B. R. (2000) The northern quahog: effect of life in a marine superfund site – New Bedford Harbor. Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 9-10, 2000.

  40. Singh, B. R., Cai, S., Li, L., Li, B., Sharma, S. K., Shukla, H. D., Lomneth, R., Sarkar, H. K. (1999) Molecular basis of the unique endopeptidase activity of botulinum neurotoxin: role of dynamic structure. Basic and Therapeutic Aspects of Botulinum and Tetanus Toxins, International Conference 1999, Orlando, FL, November 16-18, 1999.

  41. Zhou, Y. and Singh, B. R. (2000) Effect of Light on Anthocyanin Level in Cranberry. Annual Meeting of Cranberry Growers, Falmouth, MA, March 7, 2000.

  42. Zhou, Y. and Singh, B. R. (2000) Induction of Cranberry Callus. Annual Meeting of Cranberry Growers, Falmouth, MA, March 7, 2000.

  43. Ivanova, D., Agrawal , T., Li, B., Caruso, F. and Singh, B. R. (2000) Expression system for cinnamomin, an elicitin associated with cranberry root rot. Annual Meeting of Cranberry Growers, Falmouth, MA, March 7, 2000.

  44. Cai, S. and Singh, B. R. (1999) Dynamic structure of botulinum neurotoxin type A and its role in the toxico-infection process. Protein Sci. 8 (Suppl. 1) 527T.

  45. Eastwood, E. L. and Singh, B. R. (1999) In search of an organ specific quahog enzyme for biomarking and bioremediation of marine pollution. Protein Sci. 8 (Suppl. 1) 503T.

  46. Blanchette, B. N. and Singh, B. R. (1999) Quahogs, man’s sanitation engineer. Protein Sci. 8 (Suppl. 1) 500T.

  47. Puffer, E. and Singh, B. R. (1999) Structural analysis of two isoforms of SNAP-25 to determine differential neuronal communications at various stages of development. Protein Sci. 8 (Suppl. 1) 394S.

  48. Li, L., Binz, T., Niemann, H. and Singh, B. R. (1999) Probing the mechanistic role of the zinc-binding motif in the zinc-endopeptidase domain of botulinum neurotoxin type A. Protein Sci. 8 (Suppl. 1) 392M.

  49. Sardinha, G., Li, B., Singh, B. R., Sarkar, H. K. and Caruso, F. (1999) Implications of cinnamomin as the cause of root rot in cranberry plants. Protein Sci. 8 (Suppl. 1) 42S.

  50. Sharma, S. K. and Singh, B. R. (1999) Nicking-mediated enhancement in the endopeptidase activity of type E botulinum neurotoxin. Protein Sci. 8 (Suppl. 1) 383M.

  51. Lee, D., Su, T. and Singh, B. R. (1999) Inhibition of the endopeptidase activity of type A botulinum neurotoxin by sodium perborate. Protein Sci. 8 (Suppl. 1) 311S.

  52. Shukla, H. D. and Singh, B. R. (1999) Analysis of the heat shock response in Clostridium botulinum type A. Protein Sci. 8 (Suppl. 1) 195M.

  53. Sardinha, G. and Singh, B. R. (1999) Could Cinnamomin be the Cause of root rot in cranberry plants? 5th Annual Undergraduate Research, Scholarly, Creative, and Public Service Activities, Massachusetts Public System of Higher Education, Boston, April, 1999.

  54. Eastwood, E. L. and Singh, B. R. (1999) Analysis of organ specific GST in quahogs for developing a biomarker for marine pollution. 5th Annual Undergraduate Research, Scholarly, Creative, and Public Service Activities, Massachusetts Public System of Higher Education, Boston, April, 1999.

  55. Puffer, E. and Singh, B. R. (1999) Differential analysis of two isotypes of SNAP-25, involved in neuronal communications within infants and adults. 5th Annual Undergraduate Research, Scholarly, Creative, and Public Service Activities, Massachusetts Public System of Higher Education, Boston, April, 1999.

  56. Sardinha, G., Li, B., Sarkar, H. K. and Singh, B. R. (1999) Could a single fungal protein be responsible for root rot in cranberry plants? Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 3-4, 1999.

  57. Blanchette, B. N. and Singh, B. R. (1998) Quahog glutatione-S-transferase, an enzymatic informant? Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 3-4, 1999.

  58. Cai, S. and Singh, B. R. (1999) Quaternary structure analysis of botulinum neurotoxin type A and its functional role in the toxico-infection. Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 3-4, 1999.

  59. Lee, D., Singh, B. R. and Su, T. C. K. (1999) Effect of sodium perborate on light chain of botulinum/A. Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 3-4, 1999.

  60. Eastwood, E. and Singh, B. R. (1999) Tracking marine pollution levels with quahog enzyme. Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 3-4, 1999.

  61. Li, L., Binz, T., Niemann, H. and Singh, B. R. (1999) Identification of the factor that makes human, cattle and fish susceptible to botulism. Fourth Annual Sigma Xi Research Exhibit, UMass Dartmouth, March 31-April 1, 1998.

  62. Sharma, S. K. and Singh, B. R. (1999) Molecular basis of the higher toxicity of dichain type E botulinum neurotoxin compared to its single chain form. Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 3-4, 1999.

  63. Shukla, H. D. and Singh, B. R. (1999) 2-D gel analysis of the heat shock proteins of C. botulinum type A. Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 3-4, 1999.

  64. Puffer, E. and Singh, B. R. (1999) Could one protein determine difference in neuronal communications between infants and adults? Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 3-4, 1999.

  65. Yin, H. and Singh, B. R. (1999) Variation in the composition of anthocyanins during cranberry seasonal development. Fifth Annual Sigma Xi Research Exhibit, UMass Dartmouth, May 3-4, 1999.

  66. Zachariah, V., Singh, B. R. and Sardinha, G. (1999) Purification and characterization of glutathione-S-transferase from cranberry, Vaccinium macrocarpon, fruit. Annual Meeting of Cranberry Growers, Falmouth, MA, March 9, 1999.

  67. Yin, H. and Singh, B. R. (1999) Variation in the composition and of anthocyanin and flavonols during seasonal development. Annual Meeting of Cranberry Growers, Falmouth, MA, March 9, 1999.

  68.  Boulanger, Jr., R. B. and Singh, B. R. (1999) Effect of light intensity and seasonal development on the accumulation of cranberry flavonoids. Annual Meeting of Cranberry Growers, Falmouth, MA, March 9, 1999.

  69. Eastwood, E. and Singh, B. R. (1999) Organ specific GST extraction and substrate specificity in Mercenaria mercenaria. Annual Meeting of American Association of Biochemistry and Molecular Biology, May 16-20, 1999.

  70. Puffer, E. B. and Singh, B. R. (1999) Could SNAP-25 determine differential neuronal communications in infants and adults? Annual Meeting of American Association of Biochemistry and Molecular Biology, May 16-20, 1999.

  71. Fu, F.-N., Basath, D. N. and Singh, B. R. (1999) Spectroscopic analysis of low pH and lipid induced structural changes in type A botulinum neurotoxin relevant to membran channel formation and translocation. 43rd Annual meeting of the Biophysical Society, February 13-17, 1999.

  72. Singh, B. R., Cai, S., Li, B., Sharma, S. K., Li, L., Shukla, H. D., Lomneth, R. and Sarkar, H. K. (1999) A unique bacterial design of the most poisonous poison: The C. botulinum neurotoxin complex. 1998 Meeting of Interagency Botulism Research Coordinating Committee. Nov. 2-4, 1998, Fort Washington, PA.

  73. Lebeda, F. J., Montal, M., Singh, B. R. and Byrne, M. (1999) Structural characteristics of a 23-mer channel forming peptide from botulinum neurotoxin type A. 1998 Meeting of Interagency Botulism Research Coordinating Committee. Nov. 2-4, 1998, Fort Washington, PA.

  74.  Sharma, S. K. and Singh, B. R. (1998) Conformational changes in type A Clostridium botulinum Hn-33: An effect on the biological activity. FASEB J., A1350, 232.

  75. Sun, Y. and Singh, B. R. (1998) FT-IR analysis of the effect of hydrogen-deuterium exchange on protein amide III spectra. FASEB J., A1451, 817.

  76. Walsh-Sayles, N., Cai, S., Sharma, S. K., Li, L. and Singh, B. R. (1998) A complex bacterial design of the most poisonous poison. FASEB J., A1471, 934.

  77. Boulanger, Jr., R. R. and Singh, B. R. (1998) Light regulation of anthocyanin and flavonol biosynthesis in cranberry plants. Northeastern Section of ACS Undergraduate Research Symposium, Boston, MA, April 24, 1998.

  78. Sardinha, G., Li, L., Leamnson, R. and Singh, B. R. (1998) The expression of the gene for glutathione-S-transferase in transformed XL1-blue E. coli cells, enzyme purification and assay for laboratory course exercises. Fourth Annual Sigma Xi Research Exhibit, UMass Dartmouth, March 31-April 1, 1998.

  79. Blanchette, B. N. and Singh, B. R. (1998) What is there in an enzyme besides enzymatic activity? Fourth Annual Sigma Xi Research Exhibit, UMass Dartmouth, March 31-April 1, 1998.

  80. Cai, S., Sarkar, H. K. and Singh, B. R. (1998) The new function of neurotoxin associated proteins in Clostridium botulinum neurotoxin complex. Fourth Annual Sigma Xi Research Exhibit, UMass Dartmouth, March 31-April 1, 1998.

  81. Lee, D., Singh, B. R. and Su, T. C. K. (1998) Inhibition of the catalytic activity of alcohol dehydrogenase by oxidants. Fourth Annual Sigma Xi Research Exhibit, UMass Dartmouth, March 31-April 1, 1998.

  82. Li, B., Sharma, S. K., Sarkar, H. K., Caruso, F. and Singh, B. R. (1998) Molecular analysis of cranberry root rot: Phytophthora cinnamomin genes, their detection and expression. Fourth Annual Sigma Xi Research Exhibit, UMass Dartmouth, March 31-April 1, 1998.

  83. Li, L. and Singh, B. R. (1998) Identification of the factor that makes human, cattle and fish susceptible to botulism. Fourth Annual Sigma Xi Research Exhibit, UMass Dartmouth, March 31-April 1, 1998.

  84. Sharma, S. K. and Singh, B. R. (1998) Conformational stability of a hemagglutinin-33 purified from type A Clostridium botulinum. Fourth Annual Sigma Xi Research Exhibit, UMass Dartmouth, March 31-April 1, 1998.

  85. Shukla, H. D. and Singh, B. R. (1998) Identification of DnaJ like chaperone as a heat shock protein of C. botulinum type A. Fourth Annual Sigma Xi Research Exhibit, UMass Dartmouth, March 31-April 1, 1998.

  86. Sun, Y. and Singh, B. R. (1998) Use of isotope exchange and amide III infrared spectroscopy to examine tertiary structure of proteins. Fourth Annual Sigma Xi Research Exhibit, UMass Dartmouth, March 31-April 1, 1998.

  87. Walsh-Sayles, N., Cai, S., Sharma, S. K., Li, L. and Singh, B. R. (1998) A complex bacterial design of the most poisonous poison. Fourth Annual Sigma Xi Research Exhibit, UMass Dartmouth, March 31-April 1, 1998.

  88. Yin, H. and Singh, B. R. (1998) Estimation of membrane channel size of botulinum neurotoxin type A and its heavy chain. Fourth Annual Sigma Xi Research Exhibit, UMass Dartmouth, March 31-April 1, 1998.

  89. Boulanger, Jr., R. B. and Singh, B. R. (1998) Light Regulation of Flavonol and Anthocyanin Biosynthesis in Cranberry Plants. 215th National Meeting of the American Chemical Society, March 30, 1998.

  90. Li, B., Sharma, S. K., Caruso, F., Sarkar, H. K. and Singh, B. R. (1998) Phytophthora cinnamomi genes, their detection and expression. Annual Meeting of Cranberry Growers, Falmouth, MA, March 1998.

  91. Boulanger, Jr., R. B. and Singh, B. R. (1998 Light regulation of anthocyanin biosynthesis in cranberry plants through the plant photoreceptor phytochrome. Annual Meeting of Cranberry Growers, Falmouth, MA, March 1998.

  92. Singh, B. R. (1998) Overview of the IR spectroscopy of proteins. 216th National Meeting of the American Chemical Society, Boston, MA, August 23-27, 1998.

  93. Cai, S. and Singh, B. R. (1998) Conformational analysis of proteins using the amide III region of Fourier transform infrared spectroscopy. 216th National Meeting of the American Chemical Society, Boston, MA, August 23-27, 1998.

  94. Sun, Y. and Singh, B. R. (1998) Determination of deuterated protein structure using the amide III region of FT-IR spectroscopy. 216th National Meeting of the American Chemical Society, Boston, MA, August 23-27, 1998.

  95. Boulanger, Jr., R. R. and Singh, B. R. (1998) Effect of light intensity and seasonal development on the accumulation of cranberry flavonoids. 216th National Meeting of the American Chemical Society, Boston, MA, August 23-27, 1998.

  96. Zachariah, V., Singh, B. R. and Sardinha, G. (1998) Purification and characterization of glutathione-S-transferase from cranberry, Vaccinium macrocarpon, fruits. 216th National Meeting of the American Chemical Society, Boston, MA, August 23-27, 1998.

  97. Walsh-Sayles, N. and Singh, B. R. (1998) Oyster glutathione-S-transferase as a representative of multifunctional enzymes in mollusks. 216th National Meeting of the American Chemical Society, Boston, MA, August 23-27, 1998.

  98. Sardinha, G., Singh, B. R., Li, L. and Leamnson, R. (1998) Integrated laboratory course exercises: the expression of the gene for glutathione-S-transferase in transformed XL1-blu E. coli cells-enzyme purification and assay. 216th National Meeting of the American Chemical Society, Boston, MA, August 23-27, 1998.

  99. Singh, B. R. (1998) Debating the relevance of chemistry with non-science major students. 216th National Meeting of the American Chemical Society, Boston, MA, August 23-27, 1998.

  100. Blanchette, B. N. and Singh, B. R. (1998) What is there in a quahog enzyme besides enzymatic activity? 216th National Meeting of the American Chemical Society, Boston, MA, August 23-27, 1998.

  101. Cai, S., Sarkar, H. K. and Singh, B. R. (1998) New function of neurotoxin-associated proteins in Clostridium botulinum neurotoxin complex. 216th National Meeting of the American Chemical Society, Boston, MA, August 23-27, 1998.

  102. Li, L. and Singh, B. R. (1998) Isolation of synaptotagmin as a receptor for types A and E C. botulinum neurotoxin and analysis of their comparative binding. 216th National Meeting of the American Chemical Society, Boston, MA, August 23-27, 1998.

  103. Sharma, S. K. and Singh, B. R. (1998) Functional role of Hn-33: enhanced cleavage of synaptic protein SNAP-25 by C. botulinum neurotoxin type A. 216th National Meeting of the American Chemical Society, Boston, MA, August 23-27, 1998.

  104. Shukla, H. D. and Singh, B. R. (1998) Identification of a 40 kDa heat shock protein in Clostridium botulinum type A as DnaJ-like chaperone. 216th National Meeting of the American Chemical Society, Boston, MA, August 23-27, 1998.

  105. Singh, B. R., Zhang, Z., Li, B., Li, L., Fu, F. -N., Sharma, S. K., Shukla, H. D., Parikh, S. N., Qian, X., Lafontaine, C. and Sarkar, H. K. (1997) Structure-function relationship of a novel type E botulinum neurotoxin complex. 1997 Meeting of Interagency Botulism Research Coordinating Committee. Nov. 11-14, 1997, Bethesda, Maryland.

  106. Singh, B. R., Zhang, Z., Li, B., Li, L., Fu, F. -N., Sharma, S. K., Qian, X., Lafontaine, C. and Sarkar, H. K. (1997) Molecular composition and topography of neurotoxin associated proteins (NAPs) of botulinum neurotoxin complex. Second International Meeting on the Molecular Genetics and Pathogenesis of the Clostridia, Seillac, Onzain, France, June 22-25, 1997.

  107. Singh, B. R. (1997) Teaching biochemistry laboratory course by using integrated research on xenobiotic detoxifying enzyme as an example. Invited presentation at 214th National Meeting of American Chemical Society, September 7-11, 1997.

  108. Boulanger, Jr., R. B. and Singh, B. R. Singh (1997) Light regulation of anthocyanin biosynthesis in cranberry plants through the plant photoreceptor phytochrome. Protein Sci. 6 (Suppl. 2) 425T.

  109. Blanchette, B. N. and Singh, B. R. (1997) A quahog enzyme with potential for PCB remediation. Protein Sci. 6 (Suppl. 2) 351T.

  110. Zachariah, V. T. and Singh, B. R. (1997) Effect of plant greening on the enzymatic activity of glutathione-S-transferase, a detoxifying enzyme. Protein Sci. 6 (Suppl. 2) 353M.

  111. Parikh, S. N., Yang, Z., Lomneth, R. B., Sarkar, S. K. and Singh, B. R. (1997) Botulinum neurotoxin its complex form with neurotoxin associated proteins proteolyzes SNAP-25 and blocks neurotransmitter release. Protein Sci. 6 (Suppl. 2) 327T.

  112. Shukla, H. D. and Singh, B. R. (1997) Induction of heat shock proteins in Clostridium botulinum. Protein Sci. 6 (Suppl. 2) 269T.

  113. Lindo, P., Sharma, S. K., Cai, S. and Singh, B. R. (1997) Purification and biological activity of 15 kDa component of type A Clostridium botulinum neurotoxin complex. Protein Sci. 6 (Suppl. 2) 267S.

  114. Sharma, S. K. and Singh, B. R. (1997) Clostridium botulinum type A contains 33 kDa hemagglutinin in the neurotoxin complex as a possible anchor to gastrointestinal tract during food poisoning. Protein Sci. 6 (Suppl. 2) 262M.

  115. Li, L., Li, B., Parikh, S. N., Lomneth, R. B. and Singh, B. R. (1997) A novel type E Clostridium botulinum neurotoxin progenitor complex. Protein Sci. 6 (Suppl. 2) 139T.

  116. Parikh, S. N. and Singh, B. R. (1997) Comparative membrane channel activities of botulinum neurotoxin type A and E: Estimation of their membrane channel size. Protein Sci. 6 (Suppl. 2) 330T.

  117. Cai, S. and Singh, B. R. (1997) The conformational analysis of proteins using FT-IR spectroscopy. Protein Sci. 6 (Suppl 2) 326M.

  118. Singh, B. R. (1997) Development of active student participation as a method to instill favorable attitude for chemistry learning in non-science major students. Invited presentation at 29th Central Regional Meeting, Amercan Chemical Society, May 28-30, 1997.

  119. Shukla, H. D., Sharma, S. K. and Singh, B. R. (1997) Identification of a hemagglutinin present in the neurotoxin complex of type A Clostridium botulinum as a heat shock protein. 97th General Meeting, American Society for Microbiology, Miami Beach, May 4-8, 1997. K-127.

  120. Sharma, S. K., Shukla, H. D., Li, L. and Singh, B. R. (1997) A direct binding of Hn-33 with type A botulinum neurotoxin for possible role of protection against proteolysis in gastrointestinal tract. 97th General Meeting, American Society for Microbiology, Miami Beach, May 4-8, 1997. P-23.

  121. Blanchette, B. N. and Singh, B. R. (1997) A quahog enzyme with potential for PCB remediation. Third Annual Sigma Xi Research Exhibit, UMass Dartmouth, April 2-3, 1997. Also presented at the Third UMass Undergraduate Research Symposium, Amherst, MA, April 19, 1997, and at 36th annual Undergraduate Research Symposium of Northeastern Section American Chemical Society, April 26, 1997.

  122. Boulanger, Jr., R. R. and Singh, B. R. (1997) Phytochrome mediates anthocyanin biosynthesis in cranberry plants. Third Annual Sigma Xi Research Exhibit, UMass Dartmouth, April 2-3, 1997. Also presented at the Third UMass Undergraduate Research Symposium, Amherst, MA, April 19, 1997, and at 36th annual Undergraduate Research Symposium of Northeastern Section American Chemical Society, April 26, 1997.

  123. Cai, S. and Singh, B. R. (1997) Analysis of secondary structure of proteins using FT-IR spectroscopy. Third Annual Sigma Xi Research Exhibit, UMass Dartmouth, April 2-3, 1997.

  124. Li, L. and Singh, B. R. (1997) A protein receptor of botulinum neurotoxin isolated from rat brain. Third Annual Sigma Xi Research Exhibit, UMass Dartmouth, April 2-3, 1997.

  125. Li, B., Sarkar, H. K. and Singh, B. R. (1997) PCR amplification, cloning and sequencing of cinnamomin gene from Phytophthora sp. responsible for root rot in cranberry plants. Third Annual Sigma Xi Research Exhibit, UMass Dartmouth, April 2-3, 1997.

  126. Parikh, S. N. and Singh, B. R. (1997) Estimation of membrane channel size of botulinum neurotoxin type A. Third Annual Sigma Xi Research Exhibit, UMass Dartmouth, April 2-3, 1997.

  127. Sharma, S. K. and Singh, B. R. (1997) Clostridium botulinum type A contains a single hemagglutinin in the neurotoxin complex as a possible anchor to gastro-intestinal tract during food poisoning. Third Annual Sigma Xi Research Exhibit, UMass Dartmouth, April 2-3, 1997.

  128. Shukla, H. K. and Singh, B. R. (1997) Heat shock response in Clostridium botulinum type A. Third Annual Sigma Xi Research Exhibit, UMass Dartmouth, April 2-3, 1997.

  129. Yang, Z., Parikh, S. N. and Singh, B. R. (1997) Expression and purification of SNAP-25/glutathione-S-transferase fusion protein and its cleavage by botulinum neurotoxin types A and E. Third Annual Sigma Xi Research Exhibit, UMass Dartmouth, April 2-3, 1997.

  130.  Zachariah, V. T. and Singh, B. R. (1997) Plant greening and senescence adversely affect the activity of glutathione-S-transferase, a detoxifying enzyme. Third Annual Sigma Xi Research Exhibit, UMass Dartmouth, April 2-3, 1997. Also presented at the Third UMass Undergraduate Research Symposium, Amherst, MA, April 19, 1997, and at 36th annual Undergraduate Research Symposium of Northeastern Section American Chemical Society, April 26, 1997.

  131. Li, B., Qian, X. and Sarkar, H. K. and Singh, B. R. (1996) Partial nucleotide sequence of cinnamonin from Phytophthora cinnamomin pathogen of cranberry plants. Annual meeting of Cranberry Growers Association, March 1996, Taunton, MA.

  132. Singh, B. R., Li, B., Fu, F. -N., Sharma, S. K., Qian, X., Lafontaine, C. and Sarkar, H. K. (1996) Botulinum neurotoxin complex and neurotoxin associated proteins (NAPs), 1996 Meeting of Interagency Botulism Research Coordination Committee, Frederick, MD, November 6-8, 1996.

  133. Fu, F. N. and Singh, B. R. (1996) Role of zinc in the polypeptide folding of botulinum neurotoxin. International Conference Oxford on the Biomedical Aspects of Clostridium Neurotoxins, July 7-11, 1996, Oxford, UK.

  134. Holzer, R. and Singh, B. R. (1996) Botulinum neurotoxin translocation across an artificial membrane. Thirty-Fifth Undergraduate Research Symposium, Northeastern American Chemical Society, May 9, 1996.

  135. Fu, F. -N. and Singh, B. R. (1996) Role of bound zinc in the maintenance of toxic structure of botulinum neurotoxin. Sigma Xi Research Exhibit, UMass Dartmouth, April 1996.

  136. Holzer, R. and Singh, B. R. (1996) Using ELISA to study botulinum neurotoxin translocation across an artificial membrane. Sigma Xi Research Exhibit, UMass Dartmouth, April 1996.

  137. Li, L. and Singh, B. R. (1996) Low pH-induced conformational changes in synaptosomal membrane proteins: new insight into the membrane translocation of botulinum neurotoxin. Sigma Xi Research Exhibit, UMass Dartmouth, April 1996.

  138. Parikh, S. and Singh, B. R. (1996) Comparative membrane channel activities of types A and E botulinum neurotoxin. Sigma Xi Research Exhibit, UMass Dartmouth, April 1996.

  139. Li, B., Sarkar, H. K. and Singh, B. R. (1996) Construction and sequencing of the directly ustream region of Clostridium botulinum toxin binding protein. Sigma Xi Research Exhibit, UMass Dartmouth, April 1996.

  140. Fu, F. and Singh, B. R. (1995) Role of zinc binding in the toxic structure of botulinum neurotoxin. Protein Sci. 4 (suppl 2), 110.

  141. Sharma, S. K., Fu, F.-N. and Singh, B. R. (1995) Immunological properties of hemagglutinin purified from type A Clostridium botulinum. Protein Sci. 4 (suppl 2), 110.

  142. Zhang, Z. and Singh, B. R. (1995) A novel complex of type E Clostridium botulinum. Protein Sci. 4 (suppl 2), 110.

  143. Ge, J. and Singh, B. R. (1995) Physico-chemical characterization of glutathione-S-transferase purified from oyster. Protein Sci. 4 (suppl 2), 122.

  144. Singh, B. R. and Silvia, M. A. (1995) Use of optical fiber-based biosensor for the detection of botulinum neurotoxins. Protein Sci. 4 (suppl 2), 162.

  145. Li, B., Singh, B. R. and Read, D. L. (1995) Partial nucleotide sequence of the gene coding Clostridium botulinum type A neurotoxin binding protein and compare it with other strains. Protein Sci. 4 (suppl 2), 163.

  146. Li, L. and Singh, B. R. (1995) Isolation and characterization of a protein receptor for type E botulinum neurotoxin. Protein Sci. 4 (suppl 2), 94.

  147. Singh, B. R. (1995) Structure-function relationship of bacterial protein toxins. 209th National Meeting of American Chemical Society, Anaheim, CA, April 2-6, 1995.

  148. Singh, B. R. and Silvia, M. A. (1995) Detection of botulinum neurotoxins using optical fiber-based biosensor. 209th National Meeting of American Chemical Society, Anaheim, CA, April 2-6, 1995.

  149. Singh, B. R., Lopes, T. and Silvia, M. A. (1994) Role of Botulinum Complexing Proteins in the Detection of Botulinum Neurotoxins. Scientific Conference on Chemical and Biological Defense Research, Aberdeen Proving Ground, U.S.Army, Edgewood, MD, November 15-18, 1994.

  150. Singh, B. R., Poirier, M. A., Hu, Y. and Lopes, T. (1994) Use of optical fiber-based biosensor for adsorption studies of blood proteins. Gordon Research Conference on "Analytical Biosensors"; Colby Sawer College, July 17-22, 1994.

  151. Singh, B. R., Foley, J. and Lafontaine, C. L. (1994) Physico-chemical and immunological characterization of the Type E botulinum neurotoxin binding protein purified from Clostridium botulinum. (Abstract) FASEB J. 8, A1292.

  152. Ledoux, D. N., Fu, F.-N. and Singh, B. R. (1994) Identification of epitopes in type A botulinum neurotoxin. (Abstract) FASEB J. 8, A1293.

  153. Hu, Y. and Singh, B. R. (1994) Interaction of high density and low density lipoproteins to ZeSe surface and cholesterol-modified ZeSe surface studied by FT-IR/ATR spectroscopy. (Abstract) FASEB J. 8, A1293.

  154. Fu, F.-N., Ledoux, D. N. and Singh, B. R. (1994) Structural analysis of staphylococcal enterotoxin B in aqueous solution : A comparison with crystal structure. (Abstract) FASEB J. 8, A1293.

  155. Singh, B. R., Melissa A. Silvia, David N. Ledoux and Catherine Lafontaine (1993) Immunochemical properties and detection of botulinum neurotoxins. Thirty eighth Interagency Botulism Research Coordination Committee (IBRCC) meeting, November 15-17, 1993, Madison, WI.

  156. Singh, B. R. (1993) Structure-function relationship of botulinum and tetanus neurotoxins. Third Asia Pacific Congress on Animal, Plant, and Microbial Toxins, June 27-July1, 1993, Kuala Lumpur, Malaysia.

  157. Poirier, M. A. and Singh, B. R. (1993) Interaction of high density and low density lipoproteins with cholesterol and solid surfaces as determined by an optical fiber-based biosensor, American Association of Advancement of Science Annual Meeting, February 11-16, 1993, Boston, MA.

  158.  Ledoux, D. N., Be, X. and Singh, B. R. (1993) Evidence of oligomer formation by water-soluble neurotoxins of Clostridium botulinum and Clostridium tetani. American Association of Advancement of Science Annual Meeting, February 11-16, 1993, Boston, MA.

  159. Doyle, J. and Singh, B. R. (1993) Biophysical basis of channel formation in botulinum and tetanus neurotoxins.American Association of Advancement of Science Annual Meeting, February 11-16, 1993, Boston, MA.

  160. DeOliveira, D. B., Fu, F.-N. and Singh, B. R. (1993) Estimation of protein secondary structures using the amide III region of Fourier transform infrared spectroscopy. American Association of Advancement of Science Annual Meeting, February 11-16, 1993, Boston, MA.

  161. 134 Fu, F.-N. and Singh, B. R. (1993) Kinetic analysis of the interaction of botulinum neurotoxin with lipids. American Association of Advancement of Science Annual Meeting, February 11-16, 1993, Boston, MA.

  162. Singh, B. R. (1992) Immunochemical characterization of botulinum neurotoxin in its purified and complex forms. Thirty seventh Interagency Botulism Research Coordination Committee (IBRCC) meeting, December 7-9, 1992, College Park, MD.

  163. Poirier, M. A. and Singh, B. R. (1992) Interaction of cholesterol with high density lipoproteins as determined by an optical fiber-based biosensor. Sixth Symposium of The Protein Society. San Diego, CA, July 25-29, 1992.

  164. Singh, B. R. (1992) Molecular Mechanism of Action of Clostridial Neurotoxins and its Implications to Other Fields. Plenary lecture at the IVth Pan American Symposium on Animal, Plant and Microbial Toxins (International Society of Toxinology) on July 27-31, 1992 in Campinas, Brazil.

  165. Singh, B. R. (1992) Clostridial neurotoxins as a system for studying unique structure-function relationship of proteins. Round Table Discussion on "Bacterial Neurotoxins as Treasures" at the IVth Pan American Symposium on Animal, Plant and Microbial Toxins (International Society of Toxinology) on July 27-31, 1992 in Campinas, Brazil.

  166. Singh, B. R. (1992) Structure-function relationship of Clostridial neurotoxins. IVth Pan American Symposium on Animal, Plant and Microbial Toxins (International Society of Toxinology) on July 27-31, 1992 in Campinas, Brazil.

  167. Singh, B. R. (1992) Mode of Toxic Action of botulinum and tetanus neurotoxins. International Conference on Botulinum, Tetanus Neurotoxins: Neurotransmissions and Biomedical Aspects, May 11-13, 1992, Madison, WI.

  168. Singh, B. R. (1992) Molecular structure and function of botulinum and tetanus neurotoxins. International Conference on Botulinum, Tetanus Neurotoxins: Neurotransmissions and Biomedical Aspects, May 11-13, 1992, Madison, WI.

  169. Singh, B. R., Ogert, R. A., Lopes, T., Brown, J. E., Shriver-Lake, L. C. and F. S. Ligler (1992) Use of fiber-optic based biosensor for the detection of minute amount of proteins. Photochem. Photobiol. 55, 101s.

  170. Doyle, J. and Singh, B. R. (1992) Identification of membrane channel forming domains of botulinum and tetanus neurotoxins using hydrophobic moment calculations. Thirty-first Annual Undergraduate Research Symposium, Northeastern Section, American Chemical Society. April 25, 1992, Boston University, Boston, MA.

  171. Foster, K., Fu, F.-N. and Singh, B. R. (1992) Use of FT-IR spectroscopy to analyze the molecular basis of enzymatic activity of proteins in non-aqueous solvents. Thirty-first Annual Undergraduate Research Symposium, Northeastern Section, American Chemical Society. April 25, 1992, Boston University, Boston, MA.

  172. Lopes, T. and Singh, B. R. (1992) Immunosensor based detection of the botulinum type A complex. Thirty-first Annual Undergraduate Research Symposium, Northeastern Section, American Chemical Society. April 25, 1992, Boston University, Boston, MA.

  173. Poirier, M. and Singh, B. R. (1992) Binding kinetics of high density and low density lipoproteins to a cholesterol-adsorbed optical fiber. Thirty-first Annual Undergraduate Research Symposium, Northeastern Section, American Chemical Society. April 25, 1992, Boston University, Boston, MA.

  174. Ogert, R. A., Shriver-Lake, L., Brown, J. E., Singh, B. R. and Ligler, F. S. (1992) Detection of Clostridium botulinum toxin A using a fiber optic-based biosensor. Ist European Conference on Optical and Chemical Sensors and Biosensors, Grazer Congress, Graz, Austria, April 12-15, 1992.

  175. Fu, F.-N., Singh, B. R., Fuller, M. P. and Foster, K. (1992) Use of IR spectroscopy to analyze the molecular basis of enzyme activity of proteins in non-aqueous solvents. FASEB J. A212.

  176. Singh, B. R. (1991) Molecular differences between botulinum neurotoxin and its toxoid. Thitry sixth Interagency Botulism Research Coordination Committee (IBRCC) meeting, December 16-18, 1991, Philadelphia, PA.

  177. Singh, B.R. and Be, X. H. (1991) Hydrophobic moment and structure-function relationship of botulinum and tetanus neurotoxins. Presented at the Fifth Symposium of The Protein Society, Baltimore, MD, June 22-26, 1991.

  178. Singh, B. R., Fu, F. N. and Fuller, M. P. FT-IR-ATR study of proteins on surface: detectability and adsorption density. Presented at the Fifth Symposium of The Protein Society, Baltimore, MD, June 22-26, 1991.

  179. Singh, B. R. (1990) Structural analysis of botulinum and tetanus neurotoxins using CD and FT-IR spectroscopy. Presented at the Second Asia-Pacific Congress on Animal, Plant and Microbial Toxins, Banaras Hindu University, Varanasi, India, February 19-22, 1990.

  180. Singh, B. R. (1990) 'Catalytic domains' of botulinum and tetanus neurotoxins. Presented at the Second Asia-Pacific Congress on Animal, Plant and Microbial Toxins, Banaras Hindu University, Varanasi, India, February 19-22, 1990.

  181. Singh, B. R. (1990) Chromophore topography and the primary photoprocesses of phytochrome. Presented at a workshop on Recent Advances in Bioenergetic Processes, Jawaharlal Nehru University, New Delhi, India, February 22-23, 1990.

  182. Fuller, M. P., Singh, B. R. and Garry, M. C. (1990) The binding of 1-anilino naphthalene sulfonic acid and myoglobin.: an infrared study. A presentation at 1990 FACSS meeting.

  183. Singh, B. R. and DasGupta, B. R. (1989) Molecular conformations of botulinum neurotoxins. Presented at the Fourth European Workshop on Bacterial Protein Toxins, Urbino, Italy, July 3-6, 1989.

  184. Fuller, M. P. and Singh, B. R. (1989) Sampling and resolution enhancement techniques for the infrared analysis of adsorbed proteins. Seventh Intl. Conference on Fourier Transform spectroscopy, George Mason University, Fairfax, VA, U.S.A. June 19-23, 1989.

  185. Song, P. S., Singh, B. R. and Yamazaki, I. (1988) Viscosity dependence of primary photoprocesses of 124 kDa oat phytochrome studied by picosecond fluorescence spectroscopy. Photochem. Photobiol.

  186. Singh, B. R., Wasacz, F. M. and DasGupta, B. R. (1988) Structural analysis of botulinum neurotoxin types A and E. FASEB J. 2, A1750.

  187. Singh, B. R., Hong, C. B., Hahn, T. R. and Song, P. S. (1987) Binding properties of Pr and Pfr. European Symp. on Plant Photomorphogenesis, Spetes Island, Greece, August 3-9, 1987.

  188. Singh, B. R. and Song, P. S. (1987) The chromophore topography of native oat phytochrome. European Symp. on Plant Photomorphogenesis, Spetes Island, Greece, August 3-9, 1987.

  189. Song, P. S., Chai, Y. G., Kwon, T. I., Singh, B. R. and Choi, J. K. (1986) The topography and functional implications of phytochrome chromophore. Yamada Conference of Photomorphogenesis, Okazaki, Japan. October, 1986.

  190. Kwon, T. I., Chai, Y. G., Singh, B. R. and Song, P. S. (1986) Photochem. Photobiol. 43, 37s.

  191. Singh, B. R. and Song, P. S. (1985) Binding properties of 124-KD phytochrome to intact chloroplasts. Photochem. Photobiol. 41, 44s.

  192. Chai, Y. G., Huh, J. W., Singh, B. R. and Song, P.S. (1985) Fluorescence quenching probes of the tryptophan environment in intact phytochrome. Photochem. Photobiol. 41, 44s.

  193. Singh, B. R. and Singhal, G. S. (1984) Temperature-induced absorbance changes in developing barley chloroplasts. Photochem. Photobiol. 39, 21s.


     

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