BEGIN:VCALENDAR VERSION:2.0 X-WR-CALNAME:EventsCalendar PRODID:-//hacksw/handcal//NONSGML v1.0//EN CALSCALE:GREGORIAN BEGIN:VTIMEZONE TZID:America/New_York LAST-MODIFIED:20240422T053451Z TZURL:https://www.tzurl.org/zoneinfo-outlook/America/New_York X-LIC-LOCATION:America/New_York BEGIN:DAYLIGHT TZNAME:EDT TZOFFSETFROM:-0500 TZOFFSETTO:-0400 DTSTART:19700308T020000 RRULE:FREQ=YEARLY;BYMONTH=3;BYDAY=2SU END:DAYLIGHT BEGIN:STANDARD TZNAME:EST TZOFFSETFROM:-0400 TZOFFSETTO:-0500 DTSTART:19701101T020000 RRULE:FREQ=YEARLY;BYMONTH=11;BYDAY=1SU END:STANDARD END:VTIMEZONE BEGIN:VEVENT CATEGORIES:College of Engineering,Thesis/Dissertations DESCRIPTION:BMEBT MS Thesis Defense by Andrew Medeiros Date: September 8, 2 025Time: 11amLocation: TEX 219 Title: Investigation of Irisin on Osteocyte Cell Behavior Within Induced Hyperglycemic Conditions Abstract: Type 2 di abetes mellitus (T2DM) is associated with elevated fracture risk despite n ormal bone mineral density (BMD), suggesting deficits in bone quality. Adv anced glycation end-products (AGEs), formed under chronic hyperglycemia, a lter collagen cross-linking and osteocyte signaling, impairing bone remode ling. Irisin, a myokine secreted after exercise, may counteract these effe cts by improving glucose metabolism and promoting osteoblast activity, but its role in osteocyte function remains unknown. This study investigates h ow hyperglycemia and AGEs influence osteocyte behavior and tests irisin’ s potential to mitigate these effects. Using the mature osteocyte cell lin e Ocy454, we assess both cell viability under no-sugar, high-sugar, and ir isin-treated high-sugar conditions, and gene/protein expression of key bon e remodeling markers (RANKL, OPG, SOST, TNF-α, IL-6, and RAGE). We hypoth esize that high glucose will reduce osteocyte viability and upregulate pro -resorptive signals, while irisin will restore viability and promote pro-f ormative pathways. By linking AGE-driven osteocyte dysfunction to irisin ’s therapeutic potential, this work may inform novel strategies to impro ve bone quality in T2DM, addressing a critical gap in diabetic bone pathol ogy. Advisor: , Dept. of Bioengineering (lkarim@umassd.edu) Committee memb ers: Dr. Tracie Ferreira, Dept. of BioengineeringDr. Laura Hanzly, Dept. o f Bioengineering All BMEBT graduate students are encouraged to attend, and all interested parties are invited.\nEvent page: https://www.umassd.edu/e vents/cms/bmebt-ms-thesis-defense-by-andrew-medeiros.php X-ALT-DESC;FMTTYPE=text/html:

BMEBT MS Thesis Defense by Andr ew Medeiros

Date: September 8\, 2025
Time: 11am
Location: TEX 219

Title: Investigation of Irisin on Osteocyte Cell Behavior With in Induced Hyperglycemic Conditions

Abstract:

Type 2 diabetes mellitus (T2DM) is associated with elevated fracture risk despite normal bone mineral density (BMD)\, suggesting deficits in bo ne quality. Advanced glycation end-products (AGEs)\, formed under chronic hyperglycemia\, alter collagen cross-linking and osteocyte signaling\, imp airing bone remodeling. Irisin\, a myokine secreted after exercise\, may c ounteract these effects by improving glucose metabolism and promoting oste oblast activity\, but its role in osteocyte function remains unknown. This study investigates how hyperglycemia and AGEs influence osteocyte behavio r and tests irisin’s potential to mitigate these effects. Using the matu re osteocyte cell line Ocy454\, we assess both cell viability under no-sug ar\, high-sugar\, and irisin-treated high-sugar conditions\, and gene/prot ein expression of key bone remodeling markers (RANKL\, OPG\, SOST\, TNF-α \, IL-6\, and RAGE). We hypothesize that high glucose will reduce osteocyt e viability and upregulate pro-resorptive signals\, while irisin will rest ore viability and promote pro-formative pathways. By linking AGE-driven os teocyte dysfunction to irisin’s therapeutic potential\, this work may in form novel strategies to improve bone quality in T2DM\, addressing a criti cal gap in diabetic bone pathology.

Advisor: \, D ept. of Bioengineering (lkarim@umassd.edu)

Committee membe rs:
Dr. Tracie Ferreira\, Dept. of Bioengineering
Dr. Laura Hanzly\, Dept. of Bioengineering

All BMEBT graduate students are encouraged to attend\, and all interested parties are invited.

Event page: https://www.umassd.edu/events/cms/bmebt-ms-th esis-defense-by-andrew-medeiros.php

DTSTAMP:20260310T053946 DTSTART;TZID=America/New_York:20250908T110000 DTEND;TZID=America/New_York:20250909T120000 LOCATION:TEX 219 SUMMARY;LANGUAGE=en-us:BMEBT MS Thesis Defense by Andrew Medeiros UID:59cb7708f3a6bf012bfd0aa4398bc8c6@www.umassd.edu END:VEVENT END:VCALENDAR