BEGIN:VCALENDAR VERSION:2.0 X-WR-CALNAME:EventsCalendar PRODID:-//hacksw/handcal//NONSGML v1.0//EN CALSCALE:GREGORIAN BEGIN:VTIMEZONE TZID:America/New_York LAST-MODIFIED:20240422T053451Z TZURL:https://www.tzurl.org/zoneinfo-outlook/America/New_York X-LIC-LOCATION:America/New_York BEGIN:DAYLIGHT TZNAME:EDT TZOFFSETFROM:-0500 TZOFFSETTO:-0400 DTSTART:19700308T020000 RRULE:FREQ=YEARLY;BYMONTH=3;BYDAY=2SU END:DAYLIGHT BEGIN:STANDARD TZNAME:EST TZOFFSETFROM:-0400 TZOFFSETTO:-0500 DTSTART:19701101T020000 RRULE:FREQ=YEARLY;BYMONTH=11;BYDAY=1SU END:STANDARD END:VTIMEZONE BEGIN:VEVENT CATEGORIES:College of Engineering,Graduate Studies,Lectures and Seminars,Th esis/Dissertations DESCRIPTION:Title: Evaluation of extracellular vesicles released by 3D sphe roids and 2D conventional cell culture of the SKOV3 ovarian cancer cell li ne Abstract: Cancer cells communicate with the surrounding tumor microenvi ronment and establish a metastatic niche through the secretion of small ex tracellular vesicles (sEVs), which are referred to as exosomes. These exos omes play a critical role in cancer progression by transferring bioactive cargo, including proteins, lipids, mRNAs, miRNAs, and DNA fragments, to he althy cells, thereby enabling distant organ colonization and metastasis. S KOV3, a widely used human ovarian adenocarcinoma cell line, is traditional ly cultured under anchorage-dependent (2D monolayer) conditions; however, suspension culture more faithfully mimics the three-dimensional, non-adher ent microenvironment encountered in vivo, giving rise to altered cell sign alling, spheroid formation, and distinct exosome release profiles. This st udy compares the characteristics of exosomes secreted by SKOV3 cells under 2D and 3D conditions, isolated through ultrafiltration and Size Exclusion Chromatography (SEC). Scanning Transmission Electron Microscopy (STEM), D ynamic Light Scattering (DLS), Raman spectroscopy, and Flow Cytometry were used for the characterization. Multivariate analysis across FastGrow and McCoy's media as exosome collection media identified the principal compone nts governing exosome physical properties, while optimized SEC runs demons trated how Sepharose 2B and 6B columns yield exosomal fractions. Additiona l investigations included spheroid growth analysis, refractive index analy sis, storage effects, Surface-Enhanced Raman Scattering (SERS), and BCA pr otein estimation in exosomal fractions. This study presents an accessible and reproducible isolation protocol that lowers the technical barrier acro ss laboratory settings, laying the groundwork for non-invasive exosome-bas ed cancer screening.\nEvent page: https://www.umassd.edu/events/cms/bmebt- ms-thesis-defense-by-shrimathi-venugopalakrishnan.php\nEvent link: https:/ /umassd.zoom.us/j/96638190702?pwd=SYKURVzk7JtCHoW0zM4UvgNJCbHVc7.1 X-ALT-DESC;FMTTYPE=text/html:

Title:

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Evaluation of ex tracellular vesicles released by 3D spheroids and 2D conventional cell cul ture of the SKOV3 ovarian cancer cell line

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Abstract:

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Cance r cells communicate with the surrounding tumor microenvironment and establ ish a metastatic niche through the secretion of small extracellular vesicl es (sEVs)\, which are referred to as exosomes. These exosomes play a criti cal role in cancer progression by transferring bioactive cargo\, including proteins\, lipids\, mRNAs\, miRNAs\, and DNA fragments\, to healthy cells \, thereby enabling distant organ colonization and metastasis. SKOV3\, a w idely used human ovarian adenocarcinoma cell line\, is traditionally cultu red under anchorage-dependent (2D monolayer) conditions\; however\, suspen sion culture more faithfully mimics the three-dimensional\, non-adherent m icroenvironment encountered in vivo\, giving rise to altered cell signalli ng\, spheroid formation\, and distinct exosome release profiles. This stud y compares the characteristics of exosomes secreted by SKOV3 cells under 2 D and 3D conditions\, isolated through ultrafiltration and Size Exclusion Chromatography (SEC). Scanning Transmission Electron Microscopy (STEM)\, D ynamic Light Scattering (DLS)\, Raman spectroscopy\, and Flow Cytometry we re used for the characterization. Multivariate analysis across FastGrow an d McCoy's media as exosome collection media identified the principal compo nents governing exosome physical properties\, while optimized SEC runs dem onstrated how Sepharose 2B and 6B columns yield exosomal fractions. Additi onal investigations included spheroid growth analysis\, refractive index a nalysis\, storage effects\, Surface-Enhanced Raman Scattering (SERS)\, and BCA protein estimation in exosomal fractions. This study presents an acce ssible and reproducible isolation protocol that lowers the technical barri er across laboratory settings\, laying the groundwork for non-invasive exo some-based cancer screening.

Event page: https://www.umassd.edu/events/cms/bmebt-ms-thesis-defense-by-shrimathi- venugopalakrishnan.php
Event link: https://umassd.zoom.us /j/96638190702?pwd=SYKURVzk7JtCHoW0zM4UvgNJCbHVc7.1

DTSTAMP:20260418T095758 DTSTART;TZID=America/New_York:20260513T110000 DTEND;TZID=America/New_York:20260513T120000 LOCATION:Textiles 011 SUMMARY;LANGUAGE=en-us:BMEBT MS Thesis defense by Shrimathi Venugopalakrish nan UID:b07068bf448a7240a5176e91181c44e8@www.umassd.edu END:VEVENT END:VCALENDAR