BEGIN:VCALENDAR VERSION:2.0 X-WR-CALNAME:EventsCalendar PRODID:-//hacksw/handcal//NONSGML v1.0//EN CALSCALE:GREGORIAN BEGIN:VTIMEZONE TZID:America/New_York LAST-MODIFIED:20240422T053451Z TZURL:https://www.tzurl.org/zoneinfo-outlook/America/New_York X-LIC-LOCATION:America/New_York BEGIN:DAYLIGHT TZNAME:EDT TZOFFSETFROM:-0500 TZOFFSETTO:-0400 DTSTART:19700308T020000 RRULE:FREQ=YEARLY;BYMONTH=3;BYDAY=2SU END:DAYLIGHT BEGIN:STANDARD TZNAME:EST TZOFFSETFROM:-0400 TZOFFSETTO:-0500 DTSTART:19701101T020000 RRULE:FREQ=YEARLY;BYMONTH=11;BYDAY=1SU END:STANDARD END:VTIMEZONE BEGIN:VEVENT CATEGORIES:College of Engineering,Graduate Studies,Thesis/Dissertations DESCRIPTION:BMEBT MS Thesis Defense by Abid Neron Date: May 15, 2026 Time: 11 AM Location: TEX 219 Title: Investigation of the OCY454-12H Cell Line a s an In Vitro Model for Type 2 Diabetes Mellitus-Associated Bone Dysfuncti on Abstract: Type 2 diabetes mellitus (T2DM) is a globally prevalent metab olic disorder increasingly recognized for its detrimental effects on skele tal integrity. Patients with T2DM exhibit increased bone fragility, which results in a heightened risk of bone traumas, yet the underlying mechanism s remain poorly understood. Chronic hyperglycemia and the production of ad vanced glycation end-products (AGEs) are thought to play a critical role i n skeletal deterioration by activating the receptor for advanced glycation end-products (RAGE) pathway, promoting inflammation and bone resorption. Despite this association, the cellular responses of osteocytes to varying glycemic conditions have not been fully characterized in vitro. This study investigates the OCY454-12H cell line, an osteocyte-like, murine-derived conditionally immortalized model with enhanced SOST expression, as an in v itro model for T2DM-associated bone dysfunction. Cells were exposed to var ying glucose concentrations for 10 days to model hyperglycemic conditions. Then, gene and protein expression levels of key biomarkers, SOST and RAGE , were quantified using qPCR and ELISA analyses. Significant glucose-depen dent increases in gene and protein expression were identified, with 22 mM and 34 mM glucose groups most closely matching biomarker profiles reported in T2DM-associated bone dysfunction in vivo. These findings provide preli minary support for the OCY454-12H cell line as a platform for investigatin g hyperglycemia-induced osteocyte dysfunction and establish candidate gluc ose concentrations for future in vitro T2DM bone models. Advisor: Dr. Lamy a Karim, Dept. of Bioengineering (lkarim@umassd.edu) Committee Members: Dr . Tracie Ferreira, Dept. of BioengineeringDr. Laura Hanzly, Dept. of Bioen gineering All BMEBT graduate students are encouraged to attend, and all in terested parties are invited.\nEvent page: https://www.umassd.edu/events/c ms/bmebt-ms-thesis-defense-by-abid-neron.php X-ALT-DESC;FMTTYPE=text/html:

BMEBT MS Thesis Defense by Abid Neron

Date: May 15\, 2026

Time: 11 AM

Location: T EX 219

Title: Investigation of the OCY454-12H Cell Line as an In V itro Model for Type 2 Diabetes Mellitus-Associated Bone Dysfunction

\n< p>Abstract: Type 2 diabetes mellitus (T2DM) is a globally prevalent metabo lic disorder increasingly recognized for its detrimental effects on skelet al integrity. Patients with T2DM exhibit increased bone fragility\, which results in a heightened risk of bone traumas\, yet the underlying mechanis ms remain poorly understood. Chronic hyperglycemia and the production of a dvanced glycation end-products (AGEs) are thought to play a critical role in skeletal deterioration by activating the receptor for advanced glycatio n end-products (RAGE) pathway\, promoting inflammation and bone resorption . Despite this association\, the cellular responses of osteocytes to varyi ng glycemic conditions have not been fully characterized in vitro. This st udy investigates the OCY454-12H cell line\, an osteocyte-like\, murine-der ived conditionally immortalized model with enhanced SOST expression\, as a n in vitro model for T2DM-associated bone dysfunction. Cells were exposed to varying glucose concentrations for 10 days to model hyperglycemic condi tions. Then\, gene and protein expression levels of key biomarkers\, SOST and RAGE\, were quantified using qPCR and ELISA analyses. Significant gluc ose-dependent increases in gene and protein expression were identified\, w ith 22 mM and 34 mM glucose groups most closely matching biomarker profile s reported in T2DM-associated bone dysfunction in vivo. These findings pro vide preliminary support for the OCY454-12H cell line as a platform for in vestigating hyperglycemia-induced osteocyte dysfunction and establish cand idate glucose concentrations for future in vitro T2DM bone models.

Advisor: Dr. Lamya Karim\, Dept. of Bioengineering (lkarim@umassd.edu)

Committee Members:
Dr. Tracie Ferreira\, Dept. of Bioengineering
Dr. Laura Hanzly\, D ept. of Bioengineering

All BMEBT graduate students are encouraged to attend\, and all interested parties are invited.

Event page: https://www.umassd.edu/events/cms/bmebt-ms-thesis-defense-by-abid- neron.php

DTSTAMP:20260423T091749 DTSTART;TZID=America/New_York:20260515T110000 DTEND;TZID=America/New_York:20260515T120000 LOCATION:TEX 219 SUMMARY;LANGUAGE=en-us:BMEBT MS Thesis Defense by Abid Neron UID:652254dc508c46707c2dfc10ae51d10e@www.umassd.edu END:VEVENT END:VCALENDAR