BEGIN:VCALENDAR VERSION:2.0 X-WR-CALNAME:EventsCalendar PRODID:-//hacksw/handcal//NONSGML v1.0//EN CALSCALE:GREGORIAN BEGIN:VTIMEZONE TZID:America/New_York LAST-MODIFIED:20240422T053451Z TZURL:https://www.tzurl.org/zoneinfo-outlook/America/New_York X-LIC-LOCATION:America/New_York BEGIN:DAYLIGHT TZNAME:EDT TZOFFSETFROM:-0500 TZOFFSETTO:-0400 DTSTART:19700308T020000 RRULE:FREQ=YEARLY;BYMONTH=3;BYDAY=2SU END:DAYLIGHT BEGIN:STANDARD TZNAME:EST TZOFFSETFROM:-0400 TZOFFSETTO:-0500 DTSTART:19701101T020000 RRULE:FREQ=YEARLY;BYMONTH=11;BYDAY=1SU END:STANDARD END:VTIMEZONE BEGIN:VEVENT CATEGORIES:College of Engineering,Thesis/Dissertations DESCRIPTION:Biomedical Engineering & Biotechnology BMEBT MS Thesis Defense by Sean Crowley Date: June 11, 2026 Time: 12:30 PM Location: TEX 219 Title : Comparison of Ribose and Glucose for In Vitro Glycation of Human Cortica l Bone Abstract: Patients with diabetes are at a much higher risk of exper iencing bone fractures compared to non-diabetic populations. This happens despite diabetics having relatively normal or high bone mineral density, c ausing questions as to why this occurs. Past research has shown that diabe tic bone tissue has a higher prevalence of non-enzymatic glycation, a spon taneous process where the aldehydes of sugars react with ε-amino groups o n proteins in the bone matrix to eventually form advanced glycation end-pr oducts (AGEs). AGEs alter cross-links within the matrix, which can lead to stiffer tissue and deteriorated mechanical properties. High AGE accumulat ion can occur over decades, which makes it a challenge to obtain represent ative data from diabetic patients or cadaveric bone tissue in order to stu dy these mechanisms. In vitro incubation simulations have been successfull y used to reflect the process of non-enzymatic glycation in bone tissue, w ith AGE accumulating in a matter of days/weeks. These simulations normally use ribose as the extracellular sugar source. Even though glucose is more physiologically relevant, it has little documentation in its usage in the se simulations. This project investigates the effectiveness and difference s in reactions of both ribose and glucose with human cortical bone. Bone s amples were incubated with control, ribose, and glucose solutions, and wer e then tested to measure key mechanical properties as well as AGE accumula tion. Differences in results are reported, analyzed, and discussed within this thesis, providing more insight into the mechanisms of non-enzymatic g lycation’s effect on bone properties. Advisor: Dr. Lamya Karim, Dept. of Bioengineering (lkarim@umassd.edu) Committee Members: Dr. Tracie Ferreira , Dept. of Bioengineering Dr. Qinguo Fan, Dept. of Bioengineering All BMEB T graduate students are encouraged to attend, and all interested parties a re invited.\nEvent page: https://www.umassd.edu/events/cms/bmebt-ms-thesis -defense-by-sean-crowley.php X-ALT-DESC;FMTTYPE=text/html:

Biomedical Engineering & Biotec hnology

BMEBT MS Thesis Defense by Sean Crowley

Date: June 11\, 2026

Time: 12:30 PM

Location: TEX 219

Title: Comparison of Ribose and Glucose for In Vitro Glycation of Human Cortical Bone

Abstract: Patients with diabetes are at a much higher risk o f experiencing bone fractures compared to non-diabetic populations. This h appens despite diabetics having relatively normal or high bone mineral den sity\, causing questions as to why this occurs. Past research has shown th at diabetic bone tissue has a higher prevalence of non-enzymatic glycation \, a spontaneous process where the aldehydes of sugars react with ε-amino groups on proteins in the bone matrix to eventually form advanced glycati on end-products (AGEs). AGEs alter cross-links within the matrix\, which c an lead to stiffer tissue and deteriorated mechanical properties. High AGE accumulation can occur over decades\, which makes it a challenge to obtai n representative data from diabetic patients or cadaveric bone tissue in o rder to study these mechanisms. In vitro incubation simulations have been successfully used to reflect the process of non-enzymatic glycation in bon e tissue\, with AGE accumulating in a matter of days/weeks. These simulati ons normally use ribose as the extracellular sugar source. Even though glu cose is more physiologically relevant\, it has little documentation in its usage in these simulations. This project investigates the effectiveness a nd differences in reactions of both ribose and glucose with human cortical bone. Bone samples were incubated with control\, ribose\, and glucose sol utions\, and were then tested to measure key mechanical properties as well as AGE accumulation. Differences in results are reported\, analyzed\, and discussed within this thesis\, providing more insight into the mechanisms of non-enzymatic glycation’s effect on bone properties.

Advisor : Dr. Lamya Karim\, Dept. of Bioengineering (lkarim@umassd.edu)

Co mmittee Members:

Dr. Tracie Ferreira\, Dept. of Bioengineering

Dr. Qinguo Fan\, Dept. of Bioengineering

All BMEBT graduate s tudents are encouraged to attend\, and all interested parties are invited.

Event page: https://www.umassd.edu/events/cms/bmebt-m s-thesis-defense-by-sean-crowley.php

DTSTAMP:20260520T173622 DTSTART;TZID=America/New_York:20260611T123000 DTEND;TZID=America/New_York:20260611T133000 LOCATION:TEX 219 SUMMARY;LANGUAGE=en-us:BMEBT MS Thesis Defense by Sean Crowley UID:5a812580b9add859e3a657e7025612a3@www.umassd.edu END:VEVENT END:VCALENDAR