BEGIN:VCALENDAR VERSION:2.0 X-WR-CALNAME:EventsCalendar PRODID:-//hacksw/handcal//NONSGML v1.0//EN CALSCALE:GREGORIAN BEGIN:VTIMEZONE TZID:America/New_York LAST-MODIFIED:20240422T053451Z TZURL:https://www.tzurl.org/zoneinfo-outlook/America/New_York X-LIC-LOCATION:America/New_York BEGIN:DAYLIGHT TZNAME:EDT TZOFFSETFROM:-0500 TZOFFSETTO:-0400 DTSTART:19700308T020000 RRULE:FREQ=YEARLY;BYMONTH=3;BYDAY=2SU END:DAYLIGHT BEGIN:STANDARD TZNAME:EST TZOFFSETFROM:-0400 TZOFFSETTO:-0500 DTSTART:19701101T020000 RRULE:FREQ=YEARLY;BYMONTH=11;BYDAY=1SU END:STANDARD END:VTIMEZONE BEGIN:VEVENT CATEGORIES:College of Arts and Sciences,Thesis/Dissertations DESCRIPTION:Title: Cranberry Pomace Components as a Source of Anti-Inflamma tory Natural Products Advisor: Dr. Catherine Neto, Chemistry & Biochemistr y Dept. Committee Members: Dr. Shuowei Cai, Chemistry & Biochemistry Dept. & Dr. Brian Blanchette, Chemistry & Biochemistry Dept. Abstract: The Amer ican cranberry (Vaccinium macrocarpon) is a large red berry variety native to North America known for their bioactive phytochemicals and primarily c ultivated for its use in products such as juice, sauces, or other consumab les. After the processing of cranberries for their products the skins, see ds, and other raw plant material is left behind as a sidestream product ca lled pomace. Cranberries are known to contain phytochemicals such as phyto sterols, tocopherols, and most importantly for this study, triterpenoids. Based on previous work by the group, pomace contains the highest concentra tion of these triterpenoids by mass compared to other cranberry products a nd raw materials. Work has been done previously to establish the chemoprev entive and anti-inflammatory potential of cranberry extracts rich in trite rpenoids, specifically the pentacyclic triterpenoids: ursolic acid and its hydroxycinnamoyl esters. The relative contributions between the cranberry peel and flesh compared to the contribution from seeds, to the abundance of natural products and bioactivities of cranberry pomace, is under-explor ed. Ultrasound-assisted extraction was used to isolate potential bioactive compounds from whole pomace, seedless pomace, and seed only samples. Usin g vacuum liquid chromatography, fractionation of the seedless pomace extra ct was performed to isolate compounds of interest and further analyze the bioactivities of the seedless pomace extract compared to whole pomace. A f raction containing primarily hydroxycinnamoyl esters was also isolated usi ng column chromatography for further analysis of the phytochemicals’ act ivities. Characterization using UPLC-MS and GC-MS of the extracts was perf ormed to identify and quantify natural compounds such as triterpenoids, ph ytosterols, and tocopherols. With seedless pomace extracts containing a hi gher quantity of triterpenoids than whole pomace, highlighting the abundan ce of these compounds within the peel of the fruit versus seeds.  Anti-in flammatory screening was performed to determine the ability of extracts, f ractions and specific compounds in the pomace to inhibit pro-inflammatory enzyme soluble epoxide hydrolase (sEH). sEH hydrolyzes anti-inflammatory e poxyeicosatrienoic acids (EETs), derived from arachidonic acid, into mostl y bio-inactive dihydroxyeicosatrienoic acids (DHETs), which increases net inflammation in the body. Seedless pomace extracts showed a higher anti-in flammatory potential than the whole pomace counterpart. Out of the fractio ns, SPD.2 had the lowest IC50 while also being the fraction with the highe st concentration of triterpenoids and hydroxycinnamoyl esters. Correlation assessment done via multivariate analysis revealed that the hydroxycinnam oyl esters have the strongest overall association with sEH inhibition out of the compounds analyzed. All four esters individually had the highest Pe arson correlations and VIP scores, suggesting a pharmacological significan ce in the esterified forms of the triterpenoids. The quantitative and bioa ctivity results indicate that isolating cranberry pomace components, and p reparing extracts rich in ursolic and oleanolic acid and their derivatives , is a potential way to maximize the efficacy of the pomace in pharmacolog ical applications. Meeting ID: 861 5839 2178Password: JaMXyZ Meeting link: https://us05web.zoom.us/j/86158392178?pwd=CNnJeosGsAqCSinlgFWxrghZU60hbC. 1\nEvent page: https://www.umassd.edu/events/cms/9-11-26-by-jillian-a-mauk -cranberry-pomace-components-as-a-source-o.php\nEvent link: https://us05we b.zoom.us/j/86158392178?pwd=CNnJeosGsAqCSinlgFWxrghZU60hbC.1 X-ALT-DESC;FMTTYPE=text/html:

Title: Cranberry Pomace Compone nts as a Source of Anti-Inflammatory Natural Products

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Advisor: Dr. Catherine Neto\, Chemistry & Biochemistry Dept.

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Committee Members : Dr. Shuowei Cai\, Chemistry & Biochemistry Dept. & Dr. Brian Blanchette\ , Chemistry & Biochemistry Dept.

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Abstract:

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The American cr anberry (Vaccinium macrocarpon) is a large red berry variety native to Nor th America known for their bioactive phytochemicals and primarily cultivat ed for its use in products such as juice\, sauces\, or other consumables. After the processing of cranberries for their products the skins\, seeds\, and other raw plant material is left behind as a sidestream product calle d pomace. Cranberries are known to contain phytochemicals such as phytoste rols\, tocopherols\, and most importantly for this study\, triterpenoids. Based on previous work by the group\, pomace contains the highest concentr ation of these triterpenoids by mass compared to other cranberry products and raw materials. Work has been done previously to establish the chemopre ventive and anti-inflammatory potential of cranberry extracts rich in trit erpenoids\, specifically the pentacyclic triterpenoids: ursolic acid and i ts hydroxycinnamoyl esters. The relative contributions between the cranber ry peel and flesh compared to the contribution from seeds\, to the abundan ce of natural products and bioactivities of cranberry pomace\, is under-ex plored.

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Ultrasound-assisted extraction was used to isolate potenti al bioactive compounds from whole pomace\, seedless pomace\, and seed only samples. Using vacuum liquid chromatography\, fractionation of the seedle ss pomace extract was performed to isolate compounds of interest and furth er analyze the bioactivities of the seedless pomace extract compared to wh ole pomace. A fraction containing primarily hydroxycinnamoyl esters was al so isolated using column chromatography for further analysis of the phytoc hemicals’ activities. Characterization using UPLC-MS and GC-MS of the ex tracts was performed to identify and quantify natural compounds such as tr iterpenoids\, phytosterols\, and tocopherols. With seedless pomace extract s containing a higher quantity of triterpenoids than whole pomace\, highli ghting the abundance of these compounds within the peel of the fruit versu s seeds. 

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Anti-inflammatory screening was performed to determine the ability of extracts\, fractions and specific compounds in the pomace t o inhibit pro-inflammatory enzyme soluble epoxide hydrolase (sEH). sEH hyd rolyzes anti-inflammatory epoxyeicosatrienoic acids (EETs)\, derived from arachidonic acid\, into mostly bio-inactive dihydroxyeicosatrienoic acids (DHETs)\, which increases net inflammation in the body. Seedless pomace ex tracts showed a higher anti-inflammatory potential than the whole pomace c ounterpart. Out of the fractions\, SPD.2 had the lowest IC50 while also be ing the fraction with the highest concentration of triterpenoids and hydro xycinnamoyl esters. Correlation assessment done via multivariate analysis revealed that the hydroxycinnamoyl esters have the strongest overall assoc iation with sEH inhibition out of the compounds analyzed. All four esters individually had the highest Pearson correlations and VIP scores\, suggest ing a pharmacological significance in the esterified forms of the triterpe noids.

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The quantitative and bioactivity results indicate that isol ating cranberry pomace components\, and preparing extracts rich in ursolic and oleanolic acid and their derivatives\, is a potential way to maximize the efficacy of the pomace in pharmacological applications.

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Meeti ng ID: 861 5839 2178
Password: JaMXyZ

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Meeting link: https://u s05web.zoom.us/j/86158392178?pwd=CNnJeosGsAqCSinlgFWxrghZU60hbC.1

Ev ent page: https://www.umassd.edu /events/cms/9-11-26-by-jillian-a-mauk-cranberry-pomace-components-as-a-sou rce-o.php
Event link: https://us05web.zoom.us/j/86158392 178?pwd=CNnJeosGsAqCSinlgFWxrghZU60hbC.1

DTSTAMP:20260827T224838 DTSTART;TZID=America/New_York:20260911T130000 DTEND;TZID=America/New_York:20260911T150000 LOCATION:Violette 210 SUMMARY;LANGUAGE=en-us:MS Thesis Defense by Jillian A. Mauk, "Cranberry Pom ace Components as a Source of Anti-Inflammatory Natural Products" UID:1e70ff105491bef6da39e4d512bc80ef@www.umassd.edu END:VEVENT END:VCALENDAR