Biomanufacturing Lab

The CIE's Biomanufacturing Laboratory works to provide education and workforce development for biotechnology and biopharmaceutical industries, as well as support local and startup companies through the process of product development. 

Objectives

  • Offer wet lab courses such as bioengineering lab and others.
  • Support faculty research in the areas of molecular biology, biochemistry and bioengineering.
  • Provide lab and bench space, equipment, instrument and mentorship to students for their thesis research and capstone projects.
  • Provide equipment, instrument and engineering expertise for start-up companies to develop their products.

Capabilities

  • Molecular cloning and expressing genes in bacterial systems.
  • Fermentation and bioreactor design.
  • Isolation and purification of recombinant proteins.
  • Isolation and purification of plasmid DNAs.
  • Chemical analysis, biological assay and testing.

Instrument and Equipment

  • Atomic absorption spectrometer (Perkin Elmer AAnalyst 300)
  • Autoclaves (Vernitron Majestic, Napco Model 8000-DSE, Tuttnauer)
  • Balance and scale (Mettler Toledo PB 303-S, A&D FR-300) 
  • Biological safety hood (Baker SteriGard) 
  • Centrifuges (Eppendorf 5415 R, Fisher Scientific Centrific)
  • CO2 incubator (Sanyo MCO-17AIC) 
  • Coliform incubator bath (Precision Scientific)
  • Energy dispersed X-ray spectroscopy (Oxford Instruments INCA Energy)
  • Fourier transferred infrared spectrophotometer (Perkin Elmer Spectrum 100)
  • Freezer (Revco PLUS ultra-low temperature freezer)
  • Gas chromatography (Buck Scientific Model 910)
  • Gas chromatography mass spectrometer (Hewlett Packard HP 6890/MSD 5973)
  • Gel electrophoresis (Invitrogen Novex Mini-Cell)
  • Hemocytometer (Hausser Scientific Bright-Line)
  • High performance liquid chromatography (Buck Scientific BLC-20)
  • Infrared spectrophotometer (Buck Scientific Model 500) 
  • Lab ovens (Quincy Lab Inc., Sheldon Manufacturing, Inc.)
  • Optical microscopes (Olympus BH-2, Leica ATC 2000, Fisher Scientific, Edmund Industrial Optics)
  • pH meters (Cole Parmer, American Scientific) 
  • Potentiostat (Digi-Ivy DY2300)
  • Refrigerator (Kenmore)
  • Scanning electron microscope (Jeol JSM 5610) 
  • Shaker incubators (New Brunswick Sciritific Innova 2100, Thermo Scientific MaxQ 4450) 
  • Sputter coater (Denton Vacuum)
  • Ultrasonic water bath (VWR Scientific Aquasonic)
  • UV/Vis spectrophotometers (Hach DR/4000U)
  • Ventilation hoods (Fisher Hamilton SafeAire)
  • Vortex (VWR Vortexer, Scientific Industries Vortex-Genie 2)
  • Water bath (Precision Scientific)
  • Water purification system (Thermo Scientific E-pure)