CHM 514: Biochemistry Laboratory - spring

Prerequisite: CHM511 (minimum grade of C) and CHM512 (minimum grade of C; may be taken concurrently), or permission of the instructor.

Integration of modern biochemistry and molecular biology in laboratory and pre-lab lectures. Students develop fundamental laboratory skills, experimental design strategies, quantitative data analysis, and scientific communication. Techniques include spectrophotometry, gel electrophoresis, chromatography, centrifugation, PCR, molecular cloning, plasmid construction and isolation, recombinant protein expression and purification, and enzyme kinetics. Emphasis is placed on research design, data interpretation, and scientific writing. The course equips students with essential practical skills and conceptual foundations for advanced study and research in biochemistry and molecular biology.